ArticleThe EMBO journal2001
Hormone-induced nucleosome positioning in the MMTV promoter is reversible.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 52 citations in OpenAlex.
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- Oct-1 modifies S100A4 exchange between intra- and extracellular compartments in Namalwa cells and increases their sensitivity to glucocorticoids.Cell cycle (Georgetown, Tex.) · 2016Article
- Quantification of transcription factor-DNA binding affinity in a living cell.Nucleic acids research · 2016Article
- Loss of Interdependent Binding by the FoxO1 and FoxA1/A2 Forkhead Transcription Factors Culminates in Perturbation of Active Chromatin Marks and Binding of Transcriptional Regulators at Insulin-sensitive Genes.The Journal of biological chemistry · 2016Article
- The polyglutamine-expanded androgen receptor has increased DNA binding and reduced transcriptional activity.Biochemistry and biophysics reports · 2015Article
- Article
- FoxA1 binding directs chromatin structure and the functional response of a glucocorticoid receptor-regulated promoter.Molecular and cellular biology · 2009Article
- Mechanism of histone H1-stimulated glucocorticoid receptor DNA binding in vivo.Molecular and cellular biology · 2007Article
- Chromatin-mediated restriction of nuclear factor 1/CTF binding in a repressed and hormone-activated promoter in vivo.Molecular and cellular biology · 2004Article
- In vivo interactions of the Acanthamoeba TBP gene promoter.Nucleic acids research · 2004Article
- Cloning and characterization of hELD/OSA1, a novel BRG1 interacting protein.The Biochemical journal · 2002Article
- Influenza virus infection induces metallothionein gene expression in the mouse liver and lung by overlapping but distinct molecular mechanisms.Molecular and cellular biology · 2001Article
- Identification of factors mediating the developmental regulation of the early acting -3.9 kb chicken lysozyme enhancer element.Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mouse mammary tumor virus (MMTV) promoter is induced by glucocorticoid hormone via the glucocorticoid receptor (GR). The hormone-triggered effects on MMTV transcription and chromatin structure were studied in Xenopus oocytes. We previously showed that the nucleosomes organizing the MMTV promoter became translationally positioned upon hormone induction. A single GR-binding site was necessary and sufficient for the chromatin events to occur, while transcription and basal promoter elements were dispensable. Here we show that addition of the hormone antagonists RU486 or RU43044 to the previously hormone-induced MMTV promoter results in cessation of transcription and loss of chromatin remodeling and nucleosome positioning. In vivo footprinting demonstrated agonist- and RU486-induced GR binding to its DNA response element (GRE), while the other antagonist, RU43044, did not promote GR-GRE interaction. These results demonstrate that induction and maintenance of nucleosome positioning is an active process that requires constant 'pressure' of agonist-GR-recruited chromatin-modifying factor(s) rather than GR-DNA binding itself.
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