ArticleThe EMBO journal2001
SKP1-SnRK protein kinase interactions mediate proteasomal binding of a plant SCF ubiquitin ligase.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed, 261 citations in OpenAlex.
- Protist oxygen-sensing and the role of prolyl hydroxylase-regulation of E3(SCF) ubiquitin ligases.Biochimica et biophysica acta. General subjects · 2026Review
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- HOS1-mediated both ubiquitination and nuclear-import are crucial to nitrate signaling.Frontiers in molecular biosciences · 2026Article
- Dynamic ASK1 proximity networks uncover SCF-dependent and noncanonical roles in ABA and drought adaptation.bioRxiv : the preprint server for biology · 2025Article
- Glycoregulation of E3(SCF) ubiquitin ligases in unicellular eukaryotes.Glycobiology · 2025Review
- Integrative GWAS and transcriptomic analyses reveal regulatory genes controlling shoot branching in sunflower.Frontiers in plant science · 2025Article
- Exploration of structural space of Cus17 lectin with glycans using molecular docking and simulation studies.Computational and structural biotechnology journal · 2025Article
- Physiological mechanism of lodging resistance of oat stalk and analysis of transcriptome differences.Frontiers in plant science · 2025Article
- Pleiotropic regulatory locus 1 maintains actin cytoskeleton integrity and cellular homeostasis to enable Arabidopsis root growth.iScience · 2024Article
- Deciphering the regulatory role of PheSnRK genes in Moso bamboo: insights into hormonal, energy, and stress responses.BMC genomics · 2024Article
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- Triose phosphate export from chloroplasts and cellular sugar content regulate anthocyanin biosynthesis during high light acclimation.Plant communications · 2023Article
- Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.BMC genomics · 2022Article
- FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab FungusInternational journal of molecular sciences · 2022Article
- The expression and interaction proteins analysis of BjuFKF1/LKP2 in B. juncea.Molecular genetics and genomics : MGG · 2022Article
- The key clock component ZEITLUPE (ZTL) negatively regulates ABA signaling by degradation of CHLH in Arabidopsis.Frontiers in plant science · 2022Article
- Lessons from Comparison of Hypoxia Signaling in Plants and Mammals.Plants (Basel, Switzerland) · 2021Review
- Expression GWAS ofFrontiers in plant science · 2021Article
- An orphan protein of Fusarium graminearum modulates host immunity by mediating proteasomal degradation of TaSnRK1α.Nature communications · 2020Article
45 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis Snf1-related protein kinases (SnRKs) are implicated in pleiotropic regulation of metabolic, hormonal and stress responses through their interaction with the kinase inhibitor PRL1 WD-protein. Here we show that SKP1/ASK1, a conserved SCF (Skp1-cullin-F-box) ubiquitin ligase subunit, which suppresses the skp1-4 mitotic defect in yeast, interacts with the PRL1-binding C-terminal domains of SnRKs. The same SnRK domains recruit an SKP1/ASK1-binding proteasomal protein, alpha4/PAD1, which enhances the formation of a trimeric SnRK complex with SKP1/ASK1 in vitro. By contrast, PRL1 reduces the interaction of SKP1/ASK1 with SnRKs. SKP1/ASK1 is co-immunoprecipitated with a cullin SCF subunit (AtCUL1) and an SnRK kinase, but not with PRL1 from Arabidopsis cell extracts. SKP1/ASK1, cullin and proteasomal alpha-subunits show nuclear co-localization in differentiated Arabidopsis cells, and are observed in association with mitotic spindles and phragmoplasts during cell division. Detection of SnRK in purified 26S proteasomes and co-purification of epitope- tagged SKP1/ASK1 with SnRK, cullin and proteasomal alpha-subunits indicate that the observed protein interactions between SnRK, SKP1/ASK1 and alpha4/PAD1 are involved in proteasomal binding of an SCF ubiquitin ligase in Arabidopsis.
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