ArticleThe EMBO journal2001
C26-CoA-dependent ceramide synthesis of Saccharomyces cerevisiae is operated by Lag1p and Lac1p.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 279 citations in OpenAlex.
- Mannosylinositol Phosphorylceramide Biosynthesis Is Required for Cell Surface Adaptation to Ethanol Stress in Saccharomyces cerevisiae.Molecular microbiology · 2026Article
- Uncoupling of nutrient sensing and cell size control by specific defects in ceramide structure.Biology open · 2026Article
- Structural and functional dissection of a higher-order oligomerization interface in yeast ceramide synthase.Nature communications · 2026Article
- Impairment of ribosomes and DNA biosynthesis confers resistance to Inhibition of sphingolipid biosynthesis.Molecular genetics and genomics : MGG · 2026Article
- Phenotypic upregulation of hexocylceramides and ether-linked phosphocholines as markers of human extreme longevity.Aging cell · 2025Article
- Biological Importance of Complex Sphingolipids and Their Structural Diversity in Budding YeastInternational journal of molecular sciences · 2024Review
- Regulation of cellular and systemic sphingolipid homeostasis.Nature reviews. Molecular cell biology · 2024Review
- Production of structurally diverse sphingolipids by anaerobic marine bacteria in the euxinic Black Sea water column.The ISME journal · 2024Article
- Structure and mechanism of a eukaryotic ceramide synthase complex.The EMBO journal · 2023Article
- Article
- Toxoplasma ceramide synthases: Gene duplication, functional divergence, and roles in parasite fitness.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Article
- Impaired biosynthesis of ergosterol confers resistance to complex sphingolipid biosynthesis inhibitor aureobasidin A in a PDR16-dependent manner.Scientific reports · 2023Article
- A conserved mechanism determines the activity of two pivotal transcription factors that control epidermal cell differentiation in Arabidopsis thaliana.Journal of plant research · 2023Article
- The ERAD system is restricted by elevated ceramides.Science advances · 2023Article
- Contribution of specific ceramides to obesity-associated metabolic diseases.Cellular and molecular life sciences : CMLS · 2022Review
- Long-lived Humans Have a Unique Plasma Sphingolipidome.The journals of gerontology. Series A, Biological sciences and medical sciences · 2022Article
- The Ceramide Synthase Subunit Lac1 Regulates Cell Growth and Size in Fission Yeast.International journal of molecular sciences · 2021Article
- Oceanapiside, a Marine Natural Product, Targets the Sphingolipid Pathway of Fluconazole-ResistantMarine drugs · 2021Article
- Mapping bilayer thickness in the ER membrane.Science advances · 2020Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 3 countries.
Funding
Abstract
Lag1p and Lac1p are two highly homologous membrane proteins of the endoplasmic reticulum (ER). When both genes are deleted, cells cannot transport glycosylphosphatidylinositol (GPI)-anchored proteins from the ER to the Golgi at a normal rate. Here we show that microsomes or detergent extracts from lag1lac1 double mutants lack an activity transferring C26 fatty acids from C26-coenzyme A onto dihydrosphingosine or phytosphingosine. As a consequence, in intact cells, the normal ceramides and inositolphosphorylceramides are drastically reduced. lag1lac1 cells compensate for the lack of normal sphingolipids by making increased amounts of C26 fatty acids, which become incorporated into glycerophospholipids. They also contain 20- to 25-fold more free long chain bases than wild type and accumulate very large amounts of abnormally polar ceramides. They make small amounts of abnormal mild base-resistant inositolphospholipids. The lipid remodelling of GPI-anchored proteins is severely compromised in lag1lac1 double mutants since only few and mostly abnormal ceramides are incorporated into the GPI anchors. The participation of Lag1p and Lac1p in ceramide synthesis may explain their role in determining longevity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.