ArticleProceedings of the National Academy of Sciences of the United States of America2001
Regulation of the stem cell leukemia (SCL) gene: a tale of two fishes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- A network-based trans-omics approach for predicting synergistic drug combinations.Communications medicine · 2024Article
- Transcription Factor TAL1 in Erythropoiesis.Advances in experimental medicine and biology · 2024Review
- Nrf2 and Nrf2-related proteins in development and developmental toxicity: Insights from studies in zebrafish (Danio rerio).Free radical biology & medicine · 2015Review
- Shared transcription factors contribute to distinct cell fates.Transcription · 2014Article
- Impaired in vitro erythropoiesis following deletion of the Scl (Tal1) +40 enhancer is largely compensated for in vivo despite a significant reduction in expression.Molecular and cellular biology · 2013Article
- Single site-specific integration targeting coupled with embryonic stem cell differentiation provides a high-throughput alternative to in vivo enhancer analyses.Biology open · 2013Article
- Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos.Nature methods · 2009Article
- Application of Biostatistics and Bioinformatics Tools to Identify Putative Transcription Factor-Gene Regulatory Network of Ankylosing Spondylitis and Sarcoidosis.Communications in statistics: theory and methods · 2009Article
- Functional diversification of sonic hedgehog paralog enhancers identified by phylogenomic reconstruction.Genome biology · 2007Article
- Ancient duplicated conserved noncoding elements in vertebrates: a genomic and functional analysis.Genome research · 2006Article
- Transcriptional regulation of the SCL locus: identification of an enhancer that targets the primitive erythroid lineage in vivo.Molecular and cellular biology · 2005Article
- A novel approach to identifying regulatory motifs in distantly related genomes.Genome biology · 2005Article
- Uprobe: a genome-wide universal probe resource for comparative physical mapping in vertebrates.Genome research · 2005Article
- Human-zebrafish non-coding conserved elements act in vivo to regulate transcription.Nucleic acids research · 2005Article
- Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo.Genome research · 2004Article
- Analysis of multiple genomic sequence alignments: a web resource, online tools, and lessons learned from analysis of mammalian SCL loci.Genome research · 2004Article
- Fast and sensitive multiple alignment of large genomic sequences.BMC bioinformatics · 2003Article
- Fugu ESTs: new resources for transcription analysis and genome annotation.Genome research · 2003Article
- Faithful expression of a tagged Fugu WT1 protein from a genomic transgene in zebrafish: efficient splicing of pufferfish genes in zebrafish but not mice.Nucleic acids research · 2003Article
- Comparative analysis of vertebrate dystrophin loci indicate intron gigantism as a common feature.Genome research · 2003Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The stem cell leukemia (SCL) gene encodes a tissue-specific basic helix-loop-helix (bHLH) protein with a pivotal role in hemopoiesis and vasculogenesis. Several enhancers have been identified within the murine SCL locus that direct reporter gene expression to subdomains of the normal SCL expression pattern, and long-range sequence comparisons of the human and murine SCL loci have identified additional candidate enhancers. To facilitate the characterization of regulatory elements, we have sequenced and analyzed 33 kb of the SCL genomic locus from the pufferfish Fugu rubripes, a species with a highly compact genome. Although the pattern of SCL expression is highly conserved from mammals to teleost fish, the genes flanking pufferfish SCL were unrelated to those known to flank both avian and mammalian SCL genes. These data suggest that SCL regulatory elements are confined to the region between the upstream and downstream flanking genes, a region of 65 kb in human and 8.5 kb in pufferfish. Consistent with this hypothesis, the entire 33-kb pufferfish SCL locus directed appropriate expression to hemopoietic and neural tissue in transgenic zebrafish embryos, as did a 10.4-kb fragment containing the SCL gene and extending to the 5' and 3' flanking genes. These results demonstrate the power of combining the compact genome of the pufferfish with the advantages that zebrafish provide for studies of gene regulation during development. Furthermore, the pufferfish SCL locus provides a powerful tool for the manipulation of hemopoiesis and vasculogenesis in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.