ArticleThe Journal of cell biology2001
Local photorelease of caged thymosin beta4 in locomoting keratocytes causes cell turning.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 72 citations in OpenAlex.
- Low-Temperature Fabrication of Thymosin β4-Loaded Soluble Microneedles to Promote Wound Healing by Specific Binding to Downregulated Immune Regulators Vsig4 and IL22rɑ2.Advanced healthcare materials · 2026Article
- Stress fiber contraction induces cell body rotation in single keratocytes.Biophysics and physicobiology · 2025Article
- Beyond analytic solution: Analysis of FRAP experiments by spatial simulation of the forward problem.Biophysical journal · 2023Article
- Crawling, waving, inch worming, dilating, and pivoting mechanics of migrating cells: Lessons from Ken Jacobson.Biophysical journal · 2023Review
- G-actin diffusion is insufficient to achieve F-actin assembly in fast-treadmilling protrusions.Biophysical journal · 2023Article
- Celebrating the creative scientific life of Ken Jacobson.Biophysical journal · 2023Article
- [Research advances on thymosin β4 in promoting wound healing].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2022Review
- Progress on the Function and Application of Thymosin β4.Frontiers in endocrinology · 2021Review
- Recombinant Human Thymosin Beta-4 Protects against Mouse Coronavirus Infection.Mediators of inflammation · 2021Article
- Cell Mechanics at the Rear Act to Steer the Direction of Cell Migration.Cell systems · 2020Article
- Article
- Rotation of stress fibers as a single wheel in migrating fish keratocytes.Scientific reports · 2018Article
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
- Actin Turnover in Lamellipodial Fragments.Current biology : CB · 2017Article
- Thymosin β4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition.Molecular medicine reports · 2015Article
- Structural and biochemical studies of actin in complex with synthetic macrolide tail analogues.ChemMedChem · 2014Article
- There is more than one way to model an elephant. Experiment-driven modeling of the actin cytoskeleton.Biophysical journal · 2013Review
- Synthetic spatially graded Rac activation drives cell polarization and movement.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Spatial modeling of cell signaling networks.Methods in cell biology · 2012Article
- A two-step mechanism for the folding of actin by the yeast cytosolic chaperonin.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The broad aim of this work was to explore the feasibility of using light-directed perturbation techniques to study cell locomotion. Specifically, a caged form of thymosin beta4 (Tbeta4) was photoactivated in a defined local region of locomoting fish scale keratocytes and the resulting perturbation of locomotion was studied. Purified Tbeta4 was produced in an inactive form by "caging" with ([n-nitroveratryl]oxy)chlorocarbamate. In vitro spectrophotofluorometric assays indicated that caged Tbeta4 did not change the normal actin polymerization kinetics, whereas photoactivated Tbeta4 significantly inhibited actin polymerization. With an a priori knowledge of the cytoplasmic diffusion coefficient of Tbeta4 as measured by fluorescence recovery after photobleaching experiments, the rapid sequestration of actin monomers by uncaged Tbeta4 and the consequent reduction in the diffusional spread of the Tbeta4-actin complex were predicted using Virtual Cell software (developed at the Center for Biomedical Imaging Technology, University of Connecticut Health Center). These simulations demonstrated that locally photoactivating Tbeta4 in keratocytes could potentially elicit a regional locomotory response. Indeed, when caged Tbeta4 was locally photoactivated at the wings of locomoting keratocytes, specific turning about the irradiated region was observed, whereas various controls were negative. Additionally, loading of exogenous Tbeta4 into both keratocytes and fibroblasts caused very rapid disassembly of actin filaments and reduction of cellular contractility. Based on these results, a mechanical model is proposed for the turning behavior of keratocytes in response to photoreleased Tbeta4.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.