ArticleCurrent biology : CB2001
Completion of cytokinesis in C. elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex.
Article in Current biology : CB, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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100 citing papers in PubMed, 218 citations in OpenAlex.
- Outer kinetochore proteins form linear elements to regulate vesicle transport.Journal of cell science · 2026Article
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- Essential Role of COPII Proteins in Maintaining the Contractile Ring Anchoring to the Plasma Membrane during Cytokinesis inInternational journal of molecular sciences · 2024Article
- Lipid Polarization during Cytokinesis.Cells · 2022Review
- Coronin2A links actin-based endosomal processes to the EHD1 fission machinery.Molecular biology of the cell · 2022Article
- Cep55: abscission boss or assistant?Trends in cell biology · 2021Article
- Identification of GOLPH3 Partners inCells · 2021Article
- The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge.Journal of cell science · 2019Article
- Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.Molecular biology of the cell · 2018Article
- Protease dead separase inhibits chromosome segregation and RAB-11 vesicle trafficking.Cell cycle (Georgetown, Tex.) · 2017Article
- A species-specific nematocide that results in terminal embryogenesis.The Journal of experimental biology · 2017Article
- The RNA-binding protein ATX-2 regulates cytokinesis through PAR-5 and ZEN-4.Molecular biology of the cell · 2016Article
- aura (mid1ip1l) regulates the cytoskeleton at the zebrafish egg-to-embryo transition.Development (Cambridge, England) · 2016Article
- A genetic interaction map of cell cycle regulators.Molecular biology of the cell · 2016Article
- Recycling Endosomes and Viral Infection.Viruses · 2016Review
- Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesis.Journal of cell science · 2016Article
- REI-1, a Novel Rab11 GEF with a SH3BP5 domain.Communicative & integrative biology · 2016Article
40 more citing papers are in PubMed but not listed here.
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4 authors at 2 institutions in 1 country.
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Abstract
backgroundThe terminal phase of cytokinesis in eukaryotic cells involves breakage of the intercellular canal containing the spindle midzone and resealing of the daughter cells. Recent observations suggest that the spindle midzone is required for this process. In this study, we investigated the possibility that targeted secretion in the vicinity of the spindle midzone is required for the execution of the terminal phase of cytokinesis.
resultsWe inhibited secretion in early C. elegans embryos by treatment with brefeldin A (BFA). Using 4D recordings of dividing cells, we showed that BFA induced stereotyped failures in the terminal phase of cytokinesis; although the furrow ingressed normally, after a few minutes the furrow completely regressed, even though spindle midzone and midbody microtubules appeared normal. In addition, using an FM1-43 membrane probe, we found that membrane accumulated locally at the apices of the late cleavage furrows that form the persisting intercellular canals between daughter cells. However, in BFA-treated embryos this membrane accumulation did not occur, which possibly accounts for the observed cleavage failures.
conclusionsWe have shown that BFA disrupts the terminal phase of cytokinesis in the embryonic blastomeres of C. elegans. We observed that membrane accumulates at the apices of the late cleavage furrow by means of a BFA-sensitive mechanism. We suggest that this local membrane accumulation is necessary for the completion of cytokinesis and speculate that the spindle midzone region of animal cells is functionally equivalent to the phragmoplast of plants and acts to target secretion to the equatorial plane of a cleaving cell.
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