ArticleNucleic acids research2001
DNA synthesis fidelity by the reverse transcriptase of the yeast retrotransposon Ty1.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Recent Advances in Double-Strand Break-Free Kilobase-Scale Genome Editing Technologies.Biochemistry · 2023Review
- Assessment of acute myeloid leukemia molecular measurable residual disease testing in an interlaboratory study.Blood advances · 2023Article
- Methodologies for Transcript Profiling Using Long-Read Technologies.Frontiers in genetics · 2020Review
- Synthetic evolution.Nature biotechnology · 2019Review
- High-resolution mapping of DNA polymerase fidelity using nucleotide imbalances and next-generation sequencing.Nucleic acids research · 2018Article
- Article
- Targeted mutagenesis: A sniper-like diversity generator in microbial engineering.Synthetic and systems biotechnology · 2017Review
- Rate-limiting Pyrophosphate Release by HIV Reverse Transcriptase Improves Fidelity.The Journal of biological chemistry · 2016Article
- In vivo continuous evolution of genes and pathways in yeast.Nature communications · 2016Article
- The Tvv1 retrotransposon family is conserved between plant genomes separated by over 100 million years.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- The reverse transcriptase encoded by the non-LTR retrotransposon R2 is as error-prone as that encoded by HIV-1.Journal of molecular biology · 2011Article
- Article
- The reverse transcriptase of the Tf1 retrotransposon has a specific novel activity for generating the RNA self-primer that is functional in cDNA synthesis.Journal of virology · 2008Article
- The diversity of retrotransposons and the properties of their reverse transcriptases.Virus research · 2008Review
- Localization, mobility and fidelity of retrotransposed Group II introns in rRNA genes.Nucleic acids research · 2005Article
- Detection of transposable elements by their compositional bias.BMC bioinformatics · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fidelity of the yeast retrotransposon Ty1 reverse transcriptase (RT) was determined by an assay based on gel electrophoresis. Steady-state kinetics analyses of deoxyribonucleotide (dNTP) incorporation at a defined primer-template site indicate that Ty1 RT misincorporates dNTP at a frequency of 0.45 x 10(-5) for the A(t):A mispair in which dATP is misincorporated opposite a template A to 6.27 x 10(-5) for the C(t):A mispair. The G(t):G and T(t):T mispairs are formed with very low efficiency. The fidelity parameters of Ty1 RT do not depend on whether RNA or DNA are copied. Relative to lentiviral RTs (HIV-1, HIV-2 or EIAV) Ty1 RT is approximately 10-fold less error prone. Our data also show that the Ty1 RT is able to recapitulate two error-generating mechanisms: extension of mismatches and non-templated addition of nucleotides at the end of a blunt-end primer-template.
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