ArticleNucleic acids research2001
Control of directionality in integrase-mediated recombination: examination of recombination directionality factors (RDFs) including Xis and Cox proteins.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers, 1 of them a synthesis that pooled it.
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Who cites it
94 citing papers in PubMed, 1 synthesis or guideline pooled it, 179 citations in OpenAlex.
- Systematic Discovery of a New Catalogue of Tyrosine-Type Integrases in Bacterial Genomic Islands.Applied and environmental microbiology · 2023Pooled it
- A programmed reset mechanism primesScience advances · 2026Article
- In vitro properties of large serine integrase hybrids derived from ϕC31 and TG1 integrases.Nucleic acids research · 2026Article
- Integrase-On-Demand: bioprospecting integrases for targeted genomic insertion of genetic cargo.Nucleic acids research · 2026Article
- Isolation and genomic characterization of Psychrobacillus isolate L3 and bacteriophage Spoks: a new phage-host pair from Antarctic soil.BMC genomics · 2025Article
- Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix-turn-helix protein RdfS.Nucleic acids research · 2025Article
- Large serine integrases utilise scavenged phage proteins as directionality cofactors.Nucleic acids research · 2025Article
- High activity and specificity of bacteriophage cocktails against carbapenem-resistantFrontiers in microbiology · 2024Article
- Complete genome sequences of microbacterium phages Tedro and BAjuniper.Microbiology resource announcements · 2023Article
- A systematic approach to classify and characterize genomic islands driven by conjugative mobility using protein signatures.Nucleic acids research · 2023Article
- Timing of integration into the chromosome is critical for the fitness of an integrative and conjugative element and its bacterial host.PLoS genetics · 2023Article
- Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element.Acta crystallographica. Section D, Structural biology · 2022Article
- Mycobacteriophages: From Petri dish to patient.PLoS pathogens · 2022Review
- Bioinformatic and experimental characterization of SEN1998: a conserved gene carried by the Enterobacteriaceae-associated ROD21-like family of genomic islands.Scientific reports · 2022Article
- The HipAB Toxin-Antitoxin System Stabilizes a Composite Genomic Island inFrontiers in microbiology · 2022Article
- Analysis of intact prophages in genomes ofFrontiers in microbiology · 2022Article
- Archaeal tyrosine recombinases.FEMS microbiology reviews · 2021Review
- Control of the Serine Integrase Reaction: Roles of the Coiled-Coil and Helix E Regions in DNA Site Synapsis and Recombination.Journal of bacteriology · 2021Article
- Lysogeny inMicroorganisms · 2020Review
- A quantitative binding model for the Apl protein, the dual purpose recombination-directionality factor and lysis-lysogeny regulator of bacteriophage 186.Nucleic acids research · 2020Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Similarity between the DNA substrates and products of integrase-mediated site-specific recombination reactions results in a single recombinase enzyme being able to catalyze both the integration and excision reactions. The control of directionality in these reactions is achieved through a class of small accessory factors that favor one reaction while interfering with the other. These proteins, which we will refer to collectively as recombination directionality factors (RDFs), play architectural roles in reactions catalyzed by their cognate recombinases and have been identified in conjunction with both tyrosine and serine integrases. Previously identified RDFs are typically small, basic and have diverse amino acid sequences. A subset of RDFs, the cox genes, also function as transcriptional regulators. We present here a compilation of all the known RDF proteins as well as those identified through database mining that we predict to be involved in conferring recombination directionality. Analysis of this group of proteins shows that they can be grouped into distinct sub-groups based on their sequence similarities and that they are likely to have arisen from several independent evolutionary lineages. This compilation will prove useful in recognizing new proteins that confer directionality upon site-specific recombination reactions encoded by plasmids, transposons, phages and prophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.