Evidence map›Paper›PMID 11376060›Full record

ArticleJournal of clinical microbiology2001

Characterization of human cytomegalovirus strains by analysis of short tandem repeat polymorphisms.

A Walker, S J Petheram, L Ballard, J R Murph, G J Demmler, J F Bale

Open access · greenAbstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

A WalkerDepartment of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT 84113, USA.
S J Petheram
L Ballard
J R Murph
G J Demmler
J F Bale
Pediatrics and Genetics · USUniversity of Utah · USBaylor College of Medicine · USUniversity of Iowa · US

Funding

OCCUPATIONAL RISK OF CYTOMEGALOVIRUS INFECTIONR01HD022136 · NICHD · UNIVERSITY OF IOWA · PI MURPH, JODY R · 1986 to 1999
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NICHD NIH HHS HD22136
6 · The paper itself

Abstract

Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and found that a multiplexed PCR assay using primers based upon these STRs accurately maps HCMV strains. Using primers for 10 microsatellite regions, the STR profiles of 44 wild-type and 2 laboratory strains of HCMV were characterized. The results of STR analysis were compared with those for strain characterization using nucleotide sequencing and restriction fragment length polymorphism analysis. In each instance, STR analysis accurately and specifically identified strains that were indistinguishable or distinct by conventional molecular analysis. Analysis of short tandem repeats also detected polymorphisms that supported simultaneous excretion of two HCMV strains. These results indicate that STR analysis allows rapid, precise molecular characterization of HCMV strains.

Indexed as

AdultChildChild, PreschoolCytomegalovirusCytomegalovirus InfectionsDNA PrimersFemaleHumansMicrosatellite RepeatsPolymerase Chain ReactionPolymorphism, GeneticPolymorphism, Restriction Fragment LengthSequence Analysis, DNADNA Primers

Identifiers

PMID11376060
PMCPMC88114
OpenAlexW2115346083

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.