ArticleJournal of bacteriology2001
Genetic characterization and evolutionary implications of a car gene cluster in the carbazole degrader Pseudomonas sp. strain CA10.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 96 citations in OpenAlex.
- Transcriptional heterogeneity of catabolic genes on the plasmid pCAR1 causes host-specific carbazole degradation.Applied and environmental microbiology · 2024Article
- Proteolytic Maturation of the Outer MembraneApplied and environmental microbiology · 2021Article
- Complete Genome Sequence ofMicrobiology resource announcements · 2019Article
- Genetic Characterization of aFrontiers in microbiology · 2019Article
- Article
- Growth phase-dependent expression profiles of three vital H-NS family proteins encoded on the chromosome of Pseudomonas putida KT2440 and on the pCAR1 plasmid.BMC microbiology · 2017Article
- Properties, environmental fate and biodegradation of carbazole.3 Biotech · 2017Review
- Bacterial degradation of monocyclic aromatic amines.Frontiers in microbiology · 2015Review
- Carbazole angular dioxygenation and mineralization by bacteria isolated from hydrocarbon-contaminated tropical African soil.Environmental science and pollution research international · 2014Article
- Complete Genome Sequence of the Carbazole Degrader Pseudomonas resinovorans Strain CA10 (NBRC 106553).Genome announcements · 2013Article
- Genome sequences of Pseudomonas luteola XLDN4-9 and Pseudomonas stutzeri XLDN-R, two efficient carbazole-degrading strains.Journal of bacteriology · 2012Article
- Substrate-baited nanoparticles: a catch and release strategy for enzyme recognition and harvesting.Small (Weinheim an der Bergstrasse, Germany) · 2012Article
- The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5.PloS one · 2010Article
- Carbazole-degradative IncP-7 plasmid pCAR1.2 is structurally unstable in Pseudomonas fluorescens Pf0-1, which accumulates catechol, the intermediate of the carbazole degradation pathway.Applied and environmental microbiology · 2009Article
- Article
- Isolation and description of a stable carbazole-degrading microbial consortium consisting of Chryseobacterium sp. NCY and Achromobacter sp. NCW.Current microbiology · 2008Article
- Transcriptome analysis of Pseudomonas putida KT2440 harboring the completely sequenced IncP-7 plasmid pCAR1.Journal of bacteriology · 2007Article
- The Sphingomonas plasmid pCAR3 is involved in complete mineralization of carbazole.Journal of bacteriology · 2007Article
- Plasmid pCAR3 contains multiple gene sets involved in the conversion of carbazole to anthranilate.Applied and environmental microbiology · 2006Article
- Characterization of novel carbazole catabolism genes from gram-positive carbazole degrader Nocardioides aromaticivorans IC177.Applied and environmental microbiology · 2006Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleotide sequences of the 27,939-bp-long upstream and 9,448-bp-long downstream regions of the carAaAaBaBbCAc(ORF7)Ad genes of carbazole-degrading Pseudomonas sp. strain CA10 were determined. Thirty-two open reading frames (ORFs) were identified, and the car gene cluster was consequently revealed to consist of 10 genes (carAaAaBaBbCAcAdDFE) encoding the enzymes for the three-step conversion of carbazole to anthranilate and the degradation of 2-hydroxypenta-2,4-dienoate. The high identities (68 to 83%) with the enzymes involved in 3-(3-hydroxyphenyl)propionic acid degradation were observed only for CarFE. This observation, together with the fact that two ORFs are inserted between carD and carFE, makes it quite likely that the carFE genes were recruited from another locus. In the 21-kb region upstream from carAa, aromatic-ring-hydroxylating dioxygenase genes (ORF26, ORF27, and ORF28) were found. Inductive expression in carbazole-grown cells and the results of homology searching indicate that these genes encode the anthranilate 1,2-dioxygenase involved in carbazole degradation. Therefore, these ORFs were designated antABC. Four homologous insertion sequences, IS5car1 to IS5car4, were identified in the neighboring regions of car and ant genes. IS5car2 and IS5car3 constituted the putative composite transposon containing antABC. One-ended transposition of IS5car2 together with the 5' portion of antA into the region immediately upstream of carAa had resulted in the formation of IS5car1 and ORF9. In addition to the insertion sequence-dependent recombination, gene duplications and presumed gene fusion were observed. In conclusion, through the above gene rearrangement, the novel genetic structure of the car gene cluster has been constructed. In addition, it was also revealed that the car and ant gene clusters are located on the megaplasmid pCAR1.
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