ArticleBiophysical journal2001
Femtosecond dynamics of intracellular water probed with nonlinear optical Kerr effect microspectroscopy.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Water Under Molecular Crowding: From Femtosecond H-Bond Networks Dynamics to Biological Function.Sub-cellular biochemistry · 2026Review
- Probing the structure of water in individual living cells.Nature communications · 2024Article
- Slow water dynamics in dehydrated human Jurkat T cells evaluated by dielectric spectroscopy with the Bruggeman-Hanai equation.RSC advances · 2023Article
- Anomalous sub-diffusion of water in biosystems: From hydrated protein powders to concentrated protein solution to living cells.Structural dynamics (Melville, N.Y.) · 2020Article
- Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy.Nature communications · 2019Article
- The medical application of terahertz technology in non-invasive detection of cells and tissues: opportunities and challenges.RSC advances · 2019Review
- Picosecond orientational dynamics of water in living cells.Nature communications · 2017Article
- Stimulated Raman Scattering: From Bulk to Nano.Chemical reviews · 2017Article
- Invited Review Article: Pump-probe microscopy.The Review of scientific instruments · 2016Review
- Femtosecond pulse shaping enables detection of optical Kerr-effect (OKE) dynamics for molecular imaging.Optics letters · 2014Article
- The physical chemistry of cytoplasm and its influence on cell function: an update.Molecular biology of the cell · 2013Review
- Pump-probe nonlinear phase dispersion spectroscopy.Optics express · 2013Article
- Optical heterodyne-detected Raman-induced Kerr effect (OHD-RIKE) microscopy.The journal of physical chemistry. B · 2011Article
- Invited review article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy.The Review of scientific instruments · 2009Review
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3 authors.
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Abstract
A nonlinear optical Kerr effect (OKE) microscope was developed and used to elucidate the ultra-fast diffusive motions of intracellular water molecules. In the OKE microscope, a pump-induced birefringence is sensed by a delayed probe pulse within a spatially confined volume that measures 0.5 microm in the lateral direction and 4.0 microm along the axial coordinate. This microscope allows the recording of time-resolved Kerr signals, which reflect the ultra-fast structural relaxation of the liquid, exclusively from intracellular aqueous domains. Because relaxation occurs on a picosecond time scale, only local diffusive motions are probed. The microscopic OKE signal is therefore insensitive to long-time-scale hindered translational motions enforced by intracellular mechanical barriers but probes the intrinsic orientational mobility of water molecules in cells instead. The Kerr response as determined from single intact mammalian cells under physiological conditions shows a structural relaxation time of 1.35 ps, which is 1.7 times slower than the Kerr decay observed in pure water. The data indicate that the mobility of water molecules in cellular domains is moderately restricted due to the high intracellular content of proteins and solutes.
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