ArticleBiophysical journal2001
Visualization and tracking of single protein molecules in the cell nucleus.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 144 citations in OpenAlex.
- Super-Resolution Mapping and Quantification of Molecular Diffusion via Single-Molecule Displacement/Diffusivity Mapping (SMAccounts of chemical research · 2025Article
- Single molecule dynamics in a virtual cell combining a 3-dimensional matrix model with random walks.Scientific reports · 2024Article
- Similarly slow diffusion of BAM and SecYEG complexes in live E. coli cells observed with 3D spt-PALM.Biophysical journal · 2023Article
- Quantitative analysis of peroxisome tracks using a Hidden Markov Model.Scientific reports · 2023Article
- Review
- Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes.Communications biology · 2023Article
- Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5.Biophysical reports · 2022Article
- Impact of neurite alignment on organelle motion.Journal of the Royal Society, Interface · 2022Article
- Super-resolved 3D tracking of cargo transport through nuclear pore complexes.Nature cell biology · 2022Article
- A method for imaging single molecules at the plasma membrane of live cells within tissue slices.The Journal of general physiology · 2021Article
- Comparing the permeability of human and porcine small intestinal mucus for particle transport studies.Scientific reports · 2020Article
- Single-molecule displacement mapping unveils nanoscale heterogeneities in intracellular diffusivity.Nature methods · 2020Article
- Permeability of the small intestinal mucus for physiologically relevant studies: Impact of mucus location and ex vivo treatment.Scientific reports · 2019Article
- Identifying diffusive motions in single-particle trajectories on the plasma membrane via fractional time-series models.Physical review. E · 2019Article
- Conserved linear dynamics of single-molecule Brownian motion.Nature communications · 2017Article
- Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution.Nature communications · 2016Article
- Estimating the anomalous diffusion exponent for single particle tracking data with measurement errors - An alternative approach.Scientific reports · 2015Article
- Depth-of-Focus Correction in Single-Molecule Data Allows Analysis of 3D Diffusion of the Glucocorticoid Receptor in the Nucleus.PloS one · 2015Article
- Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data.Journal of the Royal Society, Interface · 2014Article
- A bottom-up approach to understanding protein layer formation at solid-liquid interfaces.Advances in colloid and interface science · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A recently developed laser fluorescence videomicroscopy method was used to determine for the first time the intranuclear trajectories of single protein molecules. Using the recombinant Escherichia coli beta-galactosidase protein P4K, labeled with an average of 4.6 ALEXA 488 chromophores per tetramer, single P4K molecules could be localized and tracked in the nuclei of permeabilized 3T3 cells at a spatial accuracy of approximately 30 nm and a time resolution of 18 ms. Our previous photobleaching measurements indicated that P4K had two fractions inside the nucleus, a larger mobile and a smaller immobile fraction. The present study supported this observation but revealed a much larger variety of mobility classes. Thus, a fraction of P4K molecules appeared to be truly immobile while another fraction was mobile but confined to very small areas. In addition, a large fraction of the P4K molecules appeared to be mobile and to move over extended distances by diffusion. However, a quantitative analysis showed that at least two subpopulations were present differing widely in diffusion coefficients. Importantly, both the diffusion coefficients and the fractions of these subpopulations were time-dependent. Our results suggest that proteins can move inside the nucleus over extended distances by diffusion. However, intranuclear protein diffusion is severely restricted, most likely by multiple association-dissociation events and/or impermeable obstacles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.