ArticleBiophysical journal2001
Detergents as probes of hydrophobic binding cavities in serum albumin and other water-soluble proteins.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation.Analytical methods : advancing methods and applications · 2023Article
- Measles and Nipah virus assembly: Specific lipid binding drives matrix polymerization.Science advances · 2022Article
- Synthesis, Characterization and Fluorescence Properties of Novel Porous Fe/ZnO Nano-Hybrid Assemblies by Using Berberis thunbergii Extract.Journal of fluorescence · 2021Article
- Binding of a Fatty Acid-Functionalized Anderson-Type Polyoxometalate to Human Serum Albumin.Inorganic chemistry · 2020Article
- Article
- Quantification of Detergents Complexed with Membrane Proteins.Scientific reports · 2017Article
- Structural and Molecular Determinants of Membrane Binding by the HIV-1 Matrix Protein.Journal of molecular biology · 2016Article
- Single molecule compression reveals intra-protein forces drive cytotoxin pore formation.eLife · 2015Article
- Article
- Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains.Protein science : a publication of the Protein Society · 2014Article
- The investigation of the binding of 6-mercaptopurine to site I on human serum albumin.The protein journal · 2012Article
- Anticancer activity and biophysical reactivity of copper complexes of 2-(benzo[d][1,3]dioxol-5-ylmethylene)-N-alkylhydrazinecarbothioamides.Inorganic chemistry communications · 2012Article
- Positive cooperative mechanistic binding of proteins at low concentrations: a comparison of poly (sodium N-undecanoyl sulfate) and sodium dodecyl sulfate.Journal of colloid and interface science · 2011Article
- Influence of the albumin concentration and temperature on the lysis of human erythrocytes by sodium dodecyl sulfate.Journal of bioenergetics and biomembranes · 2010Article
- Insights into protein-polysorbate interactions analysed by means of isothermal titration and differential scanning calorimetry.European biophysics journal : EBJ · 2009Article
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Authors and funding
5 authors.
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Abstract
As an extension of our studies on the interaction of detergents with membranes and membrane proteins, we have investigated their binding to water-soluble proteins. Anionic aliphatic compounds (dodecanoate and dodecylsulfate) were bound to serum albumin with high affinity at nine sites; related nonionic detergents (C12E8 and dodecylmaltoside) were bound at seven to eight sites, many in common with those of dodecanoate. The compounds were also bound in the hydrophobic cavity of beta-lactoglobulin, but not to ovalbumin. In addition to the generally recognized role of the Sudlow binding region II of serum albumin (localized at the IIIA subdomain) in fatty acid binding, quenching of the fluorescence intensity of tryptophan-214 by 7,8-dibromododecylmaltoside and 12-bromododecanoate also implicate the Sudlow binding region I (subdomain IIA) as a locus for binding of aliphatic compounds. Our data document the usefulness of dodecyl amphipathic compounds as probes of hydrophobic cavities in water-soluble proteins. In conjunction with recent x-ray diffraction analyses of fatty acid binding as the starting point we propose a new symmetrical binding model for the location of nine high-affinity sites on serum albumin for aliphatic compounds.
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