ArticleBiophysical journal2001
Comparison of electron paramagnetic resonance methods to determine distances between spin labels on human carbonic anhydrase II.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Tracking Copper sensing operon Repressor (CsoR) oligomerization in solution using Electron Paramagnetic Resonance spectroscopy.Protein science : a publication of the Protein Society · 2025Article
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- Pulsed EPR distance measurements in soluble proteins by site-directed spin labeling (SDSL).Current protocols in protein science · 2013Article
- Mesopores provide an amorphous state suitable for studying biomolecular structures at cryogenic temperatures.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Algorithm for selection of optimized EPR distance restraints for de novo protein structure determination.Journal of structural biology · 2011Article
- GroEL-induced topological dislocation of a substrate protein β-sheet core: a solution EPR spin-spin distance study.Journal of chemical biology · 2010Article
- Subunit organization in the TatA complex of the twin arginine protein translocase: a site-directed EPR spin labeling study.The Journal of biological chemistry · 2010Article
- Electron spin relaxation rates for semiquinones between 25 and 295K in glass-forming solvents.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2009Article
- A Proximal Bisnitroxide Initiator: Studies in Low-Temperature Nitroxide-Mediated Polymerizations.Macromolecules · 2008Article
- Nanometer distance measurements in RNA using site-directed spin labeling.Biophysical journal · 2007Article
- Measuring nanometer distances in nucleic acids using a sequence-independent nitroxide probe.Nature protocols · 2007Article
- Site-directed spin labeling measurements of nanometer distances in nucleic acids using a sequence-independent nitroxide probe.Nucleic acids research · 2006Article
- Dipolar coupling between nitroxide spin labels: the development and application of a tether-in-a-cone model.Biophysical journal · 2006Article
- Optimal bundling of transmembrane helices using sparse distance constraints.Protein science : a publication of the Protein Society · 2004Article
- Interresidual distance determination by four-pulse double electron-electron resonance in an integral membrane protein: the Na+/proline transporter PutP of Escherichia coli.Biophysical journal · 2004Article
- Impact of Chlorine Substitution on Spin Lattice Relaxation of Triarylmethyl and 1,4-Benzosemiquinone Radicals in Glass-forming Solvents between 25 and 295 K.Applied magnetic resonanceArticle
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8 authors.
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Abstract
Four doubly spin-labeled variants of human carbonic anhydrase II and corresponding singly labeled variants were prepared by site-directed spin labeling. The distances between the spin labels were obtained from continuous-wave electron paramagnetic resonance spectra by analysis of the relative intensity of the half-field transition, Fourier deconvolution of line-shape broadening, and computer simulation of line-shape changes. Distances also were determined by four-pulse double electron-electron resonance. For each variant, at least two methods were applicable and reasonable agreement between methods was obtained. Distances ranged from 7 to 24 A. The doubly spin-labeled samples contained some singly labeled protein due to incomplete labeling. The sensitivity of each of the distance determination methods to the non-interacting component was compared.
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