ArticleBiophysical journal2001
Ion concentration-dependence of rat cardiac unitary L-type calcium channel conductance.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Honeybee CaV4 has distinct permeation, inactivation, and pharmacology from homologous NaV channels.The Journal of general physiology · 2024Article
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- Ion Channel Function and Electrical Excitability in the Zona Glomerulosa: A Network Perspective on Aldosterone Regulation.Annual review of physiology · 2021Review
- Impact of IBiophysical journal · 2020Article
- Molecular basis of allosteric Orai1 channel activation by STIM1.The Journal of physiology · 2020Review
- Curcumin, a Multi-Ion Channel Blocker That Preferentially Blocks Late NaFrontiers in physiology · 2020Article
- Ca(2+) entry into neurons is facilitated by cooperative gating of clustered CaV1.3 channels.eLife · 2016Article
- Article
- Control of Ca2+ release by action potential configuration in normal and failing murine cardiomyocytes.Biophysical journal · 2010Article
- Physiologic gating properties of unitary cardiac L-type Ca2+ channels.Biochemical and biophysical research communications · 2010Article
- Ca(2+)-dependent components of inactivation of unitary cardiac L-type Ca(2+) channels.The Journal of physiology · 2010Article
- Excitation-contraction coupling gain in ventricular myocytes: insights from a parsimonious model.The Journal of physiology · 2009Article
- Complex modulation of L-type Ca(2+) current inactivation by sorcin in isolated rabbit cardiomyocytes.Pflugers Archiv : European journal of physiology · 2009Article
- Local calcium release activation by DHPR calcium channel openings in rat cardiac myocytes.The Journal of physiology · 2008Article
- The relationship between single-channel and whole-cell conductance in the T-type Ca2+ channel CaV3.1.Biophysical journal · 2008Article
- A dendrite-autonomous mechanism for direction selectivity in retinal starburst amacrine cells.PLoS biology · 2007Article
- Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator.Nature chemical biology · 2007Article
- An enquiry into metabolite domains.Biophysical journal · 2007Article
- The ionic dependence of voltage-activated inward currents in the pharyngeal muscle of Caenorhabditis elegans.Invertebrate neuroscience : IN · 2006Article
- Quantification of calcium entry at the T-tubules and surface membrane in rat ventricular myocytes.Biophysical journal · 2006Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Little is known about the native properties of unitary cardiac L-type calcium currents (i(Ca)) measured with physiological calcium (Ca) ion concentration, and their role in excitation-contraction (E-C) coupling. Our goal was to chart the concentration-dependence of unitary conductance (gamma) to physiological Ca concentration and compare it to barium ion (Ba) conductance in the absence of agonists. In isolated, K-depolarized rat myocytes, i(Ca) amplitudes were measured using cell-attached patches with 2 to 70 mM Ca or 2 to 105 mM Ba in the pipette. At 0 mV, 2 mM of Ca produced 0.12 pA, and 2 mM of Ba produced 0.19 pA unitary currents. Unitary conductance was described by a Langmuir isotherm relationship with a maximum gammaCa of 5.3 +/- 0.2 pS (n = 15), and gammaBa of 15 +/- 1 pS (n = 27). The concentration producing half-maximal gamma, Kd(gamma), was not different between Ca (1.7 +/- 0.3 mM) and Ba (1.9 +/- 0.4 mM). We found that quasi-physiological concentrations of Ca produced currents that were as easily resolvable as those obtained with the traditionally used higher concentrations. This study leads to future work on the molecular basis of E-C coupling with a physiological concentration of Ca ions permeating the Ca channel.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.