ArticleBiophysical journal2001
Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
118 citing papers in PubMed, 309 citations in OpenAlex.
- Development of ultrafast single fluorescent-molecule imaging and its application to unravel plasma membrane structure and function in live cells.Biophysics and physicobiology · 2026Article
- Long-Term Single-Molecule Tracking in Living Cells using Weak-Affinity Protein Labeling.Angewandte Chemie (International ed. in English) · 2025Article
- Single molecule tracking based drug screening.Nature communications · 2024Article
- Single molecule dynamics in a virtual cell combining a 3-dimensional matrix model with random walks.Scientific reports · 2024Article
- A Survey of Models of Cell Membranes: Toward a New Understanding of Membrane Organization.Cold Spring Harbor perspectives in biology · 2023Review
- Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging.Journal of imaging · 2023Review
- Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology.The Journal of cell biology · 2023Article
- Evolution of the Concepts of Architecture and Supramolecular Dynamics of the Plasma Membrane.Membranes · 2023Review
- Going with the membrane flow: the impact of polarized secretion on bulk plasma membrane flows.The FEBS journal · 2023Review
- Oligomerization of peripheral membrane proteins provides tunable control of cell surface polarity.Biophysical journal · 2022Article
- Characterizing locus specific chromatin structure and dynamics with correlative conventional and super-resolution imaging in living cells.Nucleic acids research · 2022Article
- Critical roles for EGFR and EGFR-HER2 clusters in EGF binding of SW620 human carcinoma cells.Journal of the Royal Society, Interface · 2022Article
- Computational model of E-cadherin clustering under force.Biophysical journal · 2021Article
- Studying structure and functions of cell membranes by single molecule biophysical techniques.Biophysics reports · 2021Article
- Intracellular transport dynamics revealed by single-particle tracking.Biophysics reports · 2021Article
- Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy.Biomedical optics express · 2021Article
- Cell patterning by secretion-induced plasma membrane flows.Science advances · 2021Article
- Cadherin puncta are interdigitated dynamic actin protrusions necessary for stable cadherin adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A computational study of co-inhibitory immune complex assembly at the interface between T cells and antigen presenting cells.PLoS computational biology · 2021Article
- Single-molecule microscopy for in-cell quantification of protein oligomeric stoichiometry.Current opinion in structural biology · 2021Review
58 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single green fluorescent protein (GFP) molecules were successfully imaged for the first time in living cells. GFP linked to the cytoplasmic carboxyl terminus of E-cadherin (E-cad-GFP) was expressed in mouse fibroblast L cells, and observed using an objective-type total internal reflection fluorescence microscope. Based on the fluorescence intensity of individual fluorescent spots, the majority of E-cad-GFP molecules on the free cell surface were found to be oligomers of various sizes, many of them greater than dimers, suggesting that oligomerization of E-cadherin takes place before its assembly at cell-cell adhesion sites. The translational diffusion coefficient of E-cad-GFP is reduced by a factor of 10 to 40 upon oligomerization. Because such large decreases in translational mobility cannot be explained solely by increases in radius upon oligomerization, an oligomerization-induced trapping model is proposed in which, when oligomers are formed, they are trapped in place due to greatly enhanced tethering and corralling effects of the membrane skeleton on oligomers (compared with monomers). The presence of many oligomers greater than dimers on the free surface suggests that these greater oligomers are the basic building blocks for the two-dimensional cell adhesion structures (adherens junctions).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.