ArticleBiology of reproduction2001
Altering intracellular pH disrupts development and cellular organization in preimplantation hamster embryos.
Article in Biology of reproduction, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 100 citations in OpenAlex.
- Reactive Oxygen Species in Embryo Development: Sources, Impacts, and Implications for In Vitro Culture Systems.Life (Basel, Switzerland) · 2026Review
- Towards homogenous multiwell plate based pH sensors using a responsive triangulenium dye and an ATTO-647 reference dye.RSC advances · 2025Article
- The Task Matters: A Comprehensive Review and Proposed Literature Score of the Effects of Chemical and Physical Parameters on Embryo Developmental Competence.Life (Basel, Switzerland) · 2023Review
- Evaluation of an automated dish preparation system for IVF and embryo culture using a mouse mode.Scientific reports · 2023Article
- How and when to measure pH in IVF culture media: validation of a portable blood gas analyzer in two IVF culture dishes for time lapse and conventional incubators.Journal of assisted reproduction and genetics · 2023Article
- Oocyte quality assessment in marine invertebrates: a novel approach by fluorescence spectroscopy.Biological research · 2022Article
- Different COJournal of assisted reproduction and genetics · 2021Observational
- Players in Mitochondrial Dynamics and Female Reproduction.Frontiers in molecular biosciences · 2021Review
- Effect of Triclosan Exposure on Developmental Competence in Parthenogenetic Porcine Embryo during Preimplantation.International journal of molecular sciences · 2020Article
- Need for choosing the ideal pH value for IVF culture media.Journal of assisted reproduction and genetics · 2020Review
- Article
- Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepodEvolutionary applications · 2016Article
- Effective vitrification and warming of porcine embryos using a pH-stable, chemically defined medium.Scientific reports · 2016Article
- In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes.PloS one · 2015Article
- Article
- Oocyte cryopreservation: searching for novel improvement strategies.Journal of assisted reproduction and genetics · 2013Review
- Biological pH buffers in IVF: help or hindrance to success.Journal of assisted reproduction and genetics · 2011Article
- Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: implications for developmental potential.Developmental biology · 2006Article
- Imaging mitochondrial organization in living primate oocytes and embryos using multiphoton microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2003Article
- Optical workstation with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities.The Review of scientific instruments · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In early cleavage stage hamster embryos, the inability to regulate intracellular pH (pHi) properly is associated with reduced developmental competence in vitro. The disruption of mitochondrial organization is also correlated with reduced development in vitro. To determine the relationship between pHi and the disruption of cytoplasmic organization, we examined the effects of altering pHi on hamster embryo development, mitochondrial distribution, and cytoskeletal organization. The weak base trimethylamine was used to increase pHi and was found to reduce embryo development and disrupt the perinuclear organization of mitochondria. The weak acid 5,5-dimethyl-2,4-oxazolinedione was used to decrease pH(i) and was also found to reduce development and disrupt the perinuclear organization of mitochondria. With either treatment, the microfilament organization was perturbed, but the microtubule cytoskeleton was not. However, the temporal progression of the disruption of mitochondrial distribution was more rapid in alkalinized embryos than acidified embryos, as revealed by two-photon imaging of living embryos. Additionally, the disruption of the microfilament network by the two treatments was not identical. The cytoplasmic disruptions observed were not due to acute toxicity of the compounds because embryos recovered developmentally when the treatment compounds were removed. These observations link ionic homeostasis, structural integrity and developmental competence in preimplantation hamster embryos.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.