ArticleThe Biochemical journal2001
Regulation by retinoic acid of acylation-stimulating protein and complement C3 in human adipocytes.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 43 citations in OpenAlex.
- Per- and polyfluoroalkyl substances (PFAS) augment adipogenesis and shift the proteome in murine 3T3-L1 adipocytes.Toxicology · 2022Article
- Acylation stimulating protein, complement C3 and lipid metabolism in ketosis-prone diabetic subjects.PloS one · 2014Article
- Understanding postprandial inflammation and its relationship to lifestyle behaviour and metabolic diseases.International journal of vascular medicine · 2012Article
- Effects of chronic metal exposure on wild fish populations revealed by high-throughput cDNA sequencing.Ecotoxicology (London, England) · 2011Article
- Serum complement C3 has a stronger association with insulin resistance than high sensitive C-reactive protein in non-diabetic Chinese.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2011Article
- The mannose-binding lectin pathway is a significant contributor to reperfusion injury in the type 2 diabetic heart.Diabetes & vascular disease research · 2009Article
- Gene expression profiling elucidates a specific role for RARgamma in the retinoic acid-induced differentiation of F9 teratocarcinoma stem cells.Biochemical pharmacology · 2008Article
- Rosiglitazone decreases postprandial production of acylation stimulating protein in type 2 diabetics.Nutrition & metabolism · 2007Article
- Thyroid status influence on adiponectin, acylation stimulating protein (ASP) and complement C3 in hyperthyroid and hypothyroid subjects.Nutrition & metabolism · 2006Article
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acylation-stimulating protein (ASP), a product of complement C3, stimulates triacylglycerol synthesis in adipocytes. Previous studies have identified transthyretin, associated with chylomicrons, as a stimulator of C3 and ASP production. Since both transthyretin and chylomicrons transport retinyl ester/retinol, our goal was to investigate whether retinoic acid (RA) could be a potential hormonal mediator of the effect. Inhibitors of protein synthesis and protein secretion eliminated the stimulatory effects of chylomicrons on both C3 and ASP production in human differentiated adipocytes, suggesting that de novo protein synthesis and secretion are both required. Incubation with chylomicrons increased C3 mRNA levels (37+/-1.5%). RA alone or with chylomicrons had a stimulatory effect on C3 production (29-fold at 16.6 nM RA) and ASP production. An RA receptor antagonist blocked stimulation of C3 mRNA and C3 secretion by both RA and chylomicrons. Finally, RA and chylomicrons activated a 1.8 kb C3-promoter-luciferase construct transfected into 3T3-F442 and 3T3-L1 cells (by 41+/-0.2% and 69+/-0.3% respectively), possibly via RA receptor half-sites identified by sequence analysis. This is the first evidence documenting stimulation by RA of the C3 gene. Thus we propose RA as a novel cellular trigger in chylomicrons that subsequently results in increased ASP production by adipocytes after a meal.
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