ArticleThe Biochemical journal2001
A surface-associated retinol- and fatty acid-binding protein (Gp-FAR-1) from the potato cyst nematode Globodera pallida: lipid binding activities, structural analysis and expression pattern.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 125 citations in OpenAlex.
- Structure-guided screening of FAR-1 antagonists with multi-stage anthelmintic activity.Antimicrobial agents and chemotherapy · 2026Article
- Characterization and ligand binding properties of a fatty acid- and retinol- binding protein (Hp-FAR-2) from Heligmosomoides polygyrus.PLoS neglected tropical diseases · 2025Article
- The immunomodulatory p43 secreted protein of Trichuris whipworm parasites is a lipid carrier that binds signalling lipids and precursors.Scientific reports · 2025Article
- A secreted fatty acid- and retinol- binding protein frombioRxiv : the preprint server for biology · 2025Article
- Nb-FAR-1: A key developmental protein affects lipid droplet accumulation and cuticle formation in Nippostrongylus brasiliensis.PLoS neglected tropical diseases · 2025Article
- Exploring the mechanisms of host-specificity of a hyperparasitic bacterium (Frontiers in cellular and infection microbiology · 2023Review
- Article
- Transcriptional response of Meloidogyne incognita to non-fumigant nematicides.Scientific reports · 2022Article
- Function of lipid binding proteins of parasitic helminths: still a long road.Parasitology research · 2022Review
- The FAR protein family of parasitic nematodes.PLoS pathogens · 2022Review
- Transcriptomic Analysis ofFrontiers in physiology · 2022Article
- A new fungus-mediated RNAi method established and used to study the fatty acid and retinol binding protein function of the plant-parasitic nematodeRNA biology · 2021Article
- Novel Functions of the Fatty Acid and Retinol Binding Protein (FAR) Gene Family Revealed by Fungus-Mediated RNAi in the Parasitic Nematode,International journal of molecular sciences · 2021Article
- Oxylipins are implicated as communication signals in tomato-root-knot nematode (Meloidogyne javanica) interaction.Scientific reports · 2021Article
- Transcriptomic Analysis of Resistant and Susceptible Responses in a New Model Root-Knot Nematode Infection System UsingFrontiers in plant science · 2021Article
- Current Insights into Migratory Endoparasitism: Deciphering the Biology, Parasitism Mechanisms, and Management Strategies of Key Migratory Endoparasitic Phytonematodes.Plants (Basel, Switzerland) · 2020Review
- Identification and Characterization of a Fatty Acid- and Retinoid-Binding Protein Gene (International journal of molecular sciences · 2019Article
- Structure and ligand binding of As-p18, an extracellular fatty acid binding protein from the eggs of a parasitic nematode.Bioscience reports · 2019Article
- Phytoparasitic Nematode Control of Plant Hormone Pathways.Plant physiology · 2019Review
- The genome of the soybean cyst nematode (Heterodera glycines) reveals complex patterns of duplications involved in the evolution of parasitism genes.BMC genomics · 2019Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Parasitic nematodes produce at least two structurally novel classes of small helix-rich retinol- and fatty-acid-binding proteins that have no counterparts in their plant or animal hosts and thus represent potential targets for new nematicides. Here we describe a protein (Gp-FAR-1) from the plant-parasitic nematode Globodera pallida, which is a member of the nematode-specific fatty-acid- and retinol-binding (FAR) family of proteins but localizes to the surface of this species, placing it in a strategic position for interaction with the host. Recombinant Gp-FAR-1 was found to bind retinol, cis-parinaric acid and the fluorophore-tagged lipids 11-(dansylamino)undecanoic acid and dansyl-D,L-alpha-amino-octanoic acid. The fluorescence emission characteristics of the dansylated analogues indicated that the entire ligand enters the binding cavity. Fluorescence competition experiments showed that Gp-FAR-1 binds fatty acids in the range C(11) to C(24), with optimal binding at C(15). Intrinsic fluorescence analysis of a mutant protein into which a tryptophan residue had been inserted supported computer-based predictions of the position of this residue at the protein's interior and possibly also at the binding site. Of direct relevance to plant defence systems was the observation that Gp-FAR-1 binds two lipids (linolenic and linoleic acids) that are precursors of plant defence compounds and the jasmonic acid signalling pathway. Moreover, Gp-FAR-1 was found to inhibit the lipoxygenase-mediated modification of these substrates in vitro. Thus not only does Gp-FAR-1 function as a broad-spectrum retinol- and fatty-acid-binding protein, the results are consistent with the idea that Gp-FAR-1 is involved in the evasion of primary host plant defence systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.