ArticleOncogene2001
Mutant K-ras enhances apoptosis in embryonic stem cells in combination with DNA damage and is associated with increased levels of p19(ARF).
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Single-cell transcriptomics and Mendelian randomization reveal LUCAT1's role in right-sided colorectal cancer risk.Frontiers in genetics · 2024Article
- Inducible Intestine-Specific Expression of krasNeoplasia (New York, N.Y.) · 2018Article
- LIN28B promotes growth and tumorigenesis of the intestinal epithelium via Let-7.Genes & development · 2013Article
- Functional interplay between the DNA-damage-response kinase ATM and ARF tumour suppressor protein in human cancer.Nature cell biology · 2013Article
- Cdk2ap2 is a novel regulator for self-renewal of murine embryonic stem cells.Stem cells and development · 2012Article
- Migfilin sensitizes cisplatin-induced apoptosis in human glioma cells in vitro.Acta pharmacologica Sinica · 2012Article
- Hsp90 Inhibitors NVP-AUY922 and NVP-BEP800 May Exert a Significant Radiosensitization on Tumor Cells along with a Cell Type-Specific Cytotoxicity.Translational oncology · 2012Article
- Activation of K-RAS by co-mutation of codons 19 and 20 is transforming.Journal of molecular signaling · 2011Article
- Mutated K-ras(Asp12) promotes tumourigenesis in Apc(Min) mice more in the large than the small intestines, with synergistic effects between K-ras and Wnt pathways.International journal of experimental pathology · 2009Article
- Bmi1 is critical for lung tumorigenesis and bronchioalveolar stem cell expansion.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The pro-apoptotic K-Ras 4A proto-oncoprotein does not affect tumorigenesis in the ApcMin/+ mouse small intestine.BMC gastroenterology · 2008Article
- Conditional expression of mutated K-ras accelerates intestinal tumorigenesis in Msh2-deficient mice.Oncogene · 2007Article
- Stem cell gene expression changes induced specifically by mutated K-ras.Gene expression · 2007Article
- Activation of sterile20-like kinase 1 in proteasome inhibitor bortezomib-induced apoptosis in oncogenic K-ras-transformed cells.Cancer research · 2006Article
- Article
- While K-ras is essential for mouse development, expression of the K-ras 4A splice variant is dispensable.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The roles of K-ras in neoplasia are not entirely understood, although there is evidence that K-ras affects susceptibility to apoptosis, modulating survival of DNA damaged cells which would otherwise be eliminated. In this study, we investigated the effects of mutant K-ras on apoptosis in vitro following DNA damage. To avoid complications resulting from mutations in other cancer-related genes and from the presence of endogenous K-ras, we derived K-ras null embryonic stem cells. Expression of either wild-type or mutant K-ras was reconstructed by stable plasmid transfection. The cell lines were treated with etoposide, cisplatin and UV radiation and apoptosis measured flow cytometrically. Mutant K-ras potentiated the effect of etoposide-derived DNA damage by increasing apoptosis, whereas absence of K-ras had the opposite effect. This pattern was similar but less marked with cisplatin, whereas UV yielded no difference in apoptosis with regard to K-ras status, suggesting that the effect of K-ras on apoptosis is dependent on the nature of the DNA damage. To investigate possible mechanisms, we examined the expression of p19(ARF) mRNA by RT-PCR. Cells expressing mutant K-ras produced elevated levels of p19(ARF) mRNA, which could link K-ras status with p53 expression and hence susceptibility to DNA damage-induced apoptosis.
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