ArticleMolecular biology of the cell2001
Regulation of myosin heavy chain expression during rat skeletal muscle development in vitro.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 62 citations in OpenAlex.
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- Nonspecific Binding Correction for Single-Cell Mass Cytometric Analysis of Autophagy and Myoblast Differentiation.Analytical chemistry · 2021Article
- Harnessing Sphingosine-1-Phosphate Signaling and Nanotopographical Cues To Regulate Skeletal Muscle Maturation and Vascularization.ACS nano · 2017Article
- Spatial Geometries of Self-Assembled Chitohexaose Monolayers Regulate Myoblast Fusion.International journal of molecular sciences · 2016Article
- Hypertrophy-Promoting Effects of Leucine Supplementation and Moderate Intensity Aerobic Exercise in Pre-Senescent Mice.Nutrients · 2016Article
- Regulation of exercise-induced fiber type transformation, mitochondrial biogenesis, and angiogenesis in skeletal muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Review
- Article
- NFAT is a nerve activity sensor in skeletal muscle and controls activity-dependent myosin switching.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Calcineurin and heat-shock proteins modulation in clenbuterol-induced hypertrophied rat skeletal muscles.Pflugers Archiv : European journal of physiology · 2004Article
- Calcineurin is not involved in some mitochondrial enzyme adaptations to endurance exercise training in rat skeletal muscle.European journal of applied physiology · 2003Article
- Altered skeletal muscle phenotypes in calcineurin Aalpha and Abeta gene-targeted mice.Molecular and cellular biology · 2003Article
- Enhancement of calcium signalling dynamics and stability by delayed modulation of the plasma-membrane calcium-ATPase in human T cells.The Journal of physiology · 2002Article
- Functional myotube formation from adult rat satellite cells in a defined serum-free system.Biotechnology progressArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Signals that determine fast- and slow-twitch phenotypes of skeletal muscle fibers are thought to stem from depolarization, with concomitant contraction and activation of calcium-dependent pathways. We examined the roles of contraction and activation of calcineurin (CN) in regulation of slow and fast myosin heavy chain (MHC) protein expression during muscle fiber formation in vitro. Myotubes formed from embryonic day 21 rat myoblasts contracted spontaneously, and approximately 10% expressed slow MHC after 12 d in culture, as seen by immunofluorescent staining. Transfection with a constitutively active form of calcineurin (CN*) increased slow MHC by 2.5-fold as determined by Western blot. This effect was attenuated 35% by treatment with tetrodotoxin and 90% by administration of the selective inhibitor of CN, cyclosporin A. Conversely, cyclosporin A alone increased fast MHC by twofold. Cotransfection with VIVIT, a peptide that selectively inhibits calcineurin-induced activation of the nuclear factor of activated T-cells, blocked the effect of CN* on slow MHC by 70% but had no effect on fast MHC. The results suggest that contractile activity-dependent expression of slow MHC is mediated largely through the CN-nuclear factor of activated T-cells pathway, whereas suppression of fast MHC expression may be independent of nuclear factor of activated T-cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.