ArticleMolecular biology of the cell2001
Dynamics of immature secretory granules: role of cytoskeletal elements during transport, cortical restriction, and F-actin-dependent tethering.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 111 citations in OpenAlex.
- Models of Intracellular Transport: Contradictions and Current Understanding.Sub-cellular biochemistry · 2026Review
- Paracrine FGF1 signaling directs pituitary architecture and size.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Multiple pathways and independent functional pools in insulin granule exocytosis.Genes to cells : devoted to molecular & cellular mechanisms · 2023Review
- Forty years of the adrenal chromaffin cell through ISCCB meetings around the world.Pflugers Archiv : European journal of physiology · 2023Review
- The Regulated Secretion and Models of Intracellular Transport: The Goblet Cell as an Example.International journal of molecular sciences · 2023Review
- Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis.eLife · 2023Article
- Cell-specific secretory granule sorting mechanisms: the role of MAGEL2 and retromer in hypothalamic regulated secretion.Frontiers in cell and developmental biology · 2023Review
- In vivo Roles of Rab27 and Its Effectors in Exocytosis.Cell structure and function · 2021Review
- 3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells.The Journal of cell biology · 2021Article
- Determination of secretory granule maturation times in pancreatic islet β-cells by serial block-face electron microscopy.Journal of structural biology · 2020Article
- How does the stimulus define exocytosis in adrenal chromaffin cells?Pflugers Archiv : European journal of physiology · 2018Review
- Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer.Scientific reports · 2015Article
- Article
- Neuronal porosome proteome: Molecular dynamics and architecture.Journal of proteomics · 2012Article
- 3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex.Histochemistry and cell biology · 2012Article
- Myrip couples the capture of secretory granules by the actin-rich cell cortex and their attachment to the plasma membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Partitioning and Exocytosis of Secretory Granules during Division of PC12 Cells.International journal of cell biology · 2012Article
- Exophilin8 transiently clusters insulin granules at the actin-rich cell cortex prior to exocytosis.Molecular biology of the cell · 2011Article
- A distinct trans-Golgi network subcompartment for sorting of synaptic and granule proteins in neurons and neuroendocrine cells.Journal of cell science · 2011Article
- Myosin VI and its binding partner optineurin are involved in secretory vesicle fusion at the plasma membrane.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secretory granules store neuropeptides and hormones and exhibit regulated exocytosis upon appropriate cellular stimulation. They are generated in the trans-Golgi network as immature secretory granules, short-lived vesicular intermediates, which undergo a complex and poorly understood maturation process. Due to their short half-life and low abundance, real-time studies of immature secretory granules have not been previously possible. We describe here a pulse/chase-like system based on the expression of a human chromogranin B-GFP fusion protein in neuroendocrine PC12 cells, which permits direct visualization of the budding of immature secretory granules and their dynamics during maturation. Live cell imaging revealed that newly formed immature secretory granules are transported in a direct and microtubule-dependent manner within a few seconds to the cell periphery. Our data suggest that the cooperative action of microtubules and actin filaments restricts immature secretory granules to the F-actin-rich cell cortex, where they move randomly and mature completely within a few hours. During this maturation period, secretory granules segregate into pools of different motility. In a late phase of maturation, 60% of secretory granules were found to be immobile and about half of these underwent F-actin-dependent tethering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.