ArticleMolecular biology of the cell2001
Isolation and characterization of effector-loop mutants of CDC42 in yeast.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- Control of stress-activated Cdc42 dynamics by the MAP kinase Sty1-NDR kinase Orb6 regulatory axis.iScience · 2025Article
- Septin Organization and Dynamics for Budding Yeast Cytokinesis.Journal of fungi (Basel, Switzerland) · 2024Review
- Orientation of Cell Polarity by Chemical Gradients.Annual review of biophysics · 2022Review
- Cla4p Kinase Activity Is Down-Regulated by Fus3p during Yeast Mating.Biomolecules · 2022Article
- Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway inJournal of cell science · 2020Article
- Functions for Cdc42p BEM adaptors in regulating a differentiation-type MAP kinase pathway.Molecular biology of the cell · 2020Article
- Cell-cycle control of cell polarity in yeast.The Journal of cell biology · 2019Article
- Principles that govern competition or co-existence in Rho-GTPase driven polarization.PLoS computational biology · 2018Article
- Septins and Generation of Asymmetries in Fungal Cells.Annual review of microbiology · 2015Review
- Morphogenesis and the cell cycle.Genetics · 2012Review
- A switch I mutant of Cdc42 exhibits less conformational freedom.Biochemistry · 2011Article
- Dual modes of cdc42 recycling fine-tune polarized morphogenesis.Developmental cell · 2009Article
- The rate of cell growth is governed by cell cycle stage.Genes & development · 2009Article
- Effector proteins exert an important influence on the signaling-active state of the small GTPase Cdc42.The Journal of biological chemistry · 2008Article
- Identification of novel membrane-binding domains in multiple yeast Cdc42 effectors.Molecular biology of the cell · 2007Article
- Shs1 plays separable roles in septin organization and cytokinesis in Saccharomyces cerevisiae.Genetics · 2007Article
- Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle.Molecular biology of the cell · 2007Article
- Central roles of small GTPases in the development of cell polarity in yeast and beyond.Microbiology and molecular biology reviews : MMBR · 2007Review
- Role of Cdc42-Cla4 interaction in the pheromone response of Saccharomyces cerevisiae.Eukaryotic cell · 2007Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The highly conserved small GTPase Cdc42p is a key regulator of cell polarity and cytoskeletal organization in eukaryotic cells. Multiple effectors of Cdc42p have been identified, although it is unclear how their activities are coordinated to produce particular cell behaviors. One strategy used to address the contributions made by different effector pathways downstream of small GTPases has been the use of "effector-loop" mutants of the GTPase that selectively impair only a subset of effector pathways. We now report the generation and preliminary characterization of a set of effector-loop mutants of Saccharomyces cerevisiae CDC42. These mutants define genetically separable pathways influencing actin or septin organization. We have characterized the phenotypic defects of these mutants and the binding defects of the encoded proteins to known yeast Cdc42p effectors in vitro. The results suggest that these effectors cannot account for the observed phenotypes, and therefore that unknown effectors exist that affect both actin and septin organization. The availability of partial function alleles of CDC42 in a genetically tractable system serves as a useful starting point for genetic approaches to identify such novel effectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.