ArticleMolecular biology of the cell2001
UV-induced hyperphosphorylation of replication protein a depends on DNA replication and expression of ATM protein.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 90 citations in OpenAlex.
- Trafficking of hormones and trophic factors to secretory and extracellular vesicles: a historical perspective and new hypothesis.Extracellular vesicles and circulating nucleic acids · 2023Article
- Replication Protein A Phosphorylation Facilitates RAD52-Dependent Homologous Recombination in BRCA-Deficient Cells.Molecular and cellular biology · 2022Article
- hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage.Scientific reports · 2021Article
- Huaier extract restrains the proliferative potential of endocrine-resistant breast cancer cells through increased ATM by suppressing miR-203.Scientific reports · 2017Article
- Recent advancements in the discovery of protein-protein interaction inhibitors of replication protein A.MedChemComm · 2017Review
- RPA inhibition increases replication stress and suppresses tumor growth.Cancer research · 2014Article
- Article
- Interplay of DNA damage and cell cycle signaling at the level of human replication protein A.DNA repair · 2014Article
- DNA polymerase η modulates replication fork progression and DNA damage responses in platinum-treated human cells.Scientific reports · 2013Article
- Viral interference with DNA repair by targeting of the single-stranded DNA binding protein RPA.PLoS pathogens · 2013Article
- Human single-stranded DNA binding proteins are essential for maintaining genomic stability.BMC molecular biology · 2013Review
- Rfa2 is specifically dephosphorylated by Pph3 in Candida albicans.The Biochemical journal · 2013Article
- Quantitative profiling of DNA damage and apoptotic pathways in UV damaged cells using PTMScan Direct.International journal of molecular sciences · 2012Article
- Distinct roles for DNA-PK, ATM and ATR in RPA phosphorylation and checkpoint activation in response to replication stress.Nucleic acids research · 2012Article
- Replication-mediated disassociation of replication protein A-XPA complex upon DNA damage: implications for RPA handing off.Cell biology international · 2012Article
- The structure and function of replication protein A in DNA replication.Sub-cellular biochemistry · 2012Review
- Pph3 dephosphorylation of Rad53 is required for cell recovery from MMS-induced DNA damage in Candida albicans.PloS one · 2012Article
- Molecular cooperation between the Werner syndrome protein and replication protein A in relation to replication fork blockage.The Journal of biological chemistry · 2011Article
- Replication protein A: directing traffic at the intersection of replication and repair.Frontiers in bioscience (Landmark edition) · 2010Review
- Hyperphosphorylation of replication protein A in cisplatin-resistant and -sensitive head and neck squamous cell carcinoma cell lines.Head & neck · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Exposure to DNA-damaging agents triggers signal transduction pathways that are thought to play a role in maintenance of genomic stability. A key protein in the cellular processes of nucleotide excision repair, DNA recombination, and DNA double-strand break repair is the single-stranded DNA binding protein, RPA. We showed previously that the p34 subunit of RPA becomes hyperphosphorylated as a delayed response (4-8 h) to UV radiation (10-30 J/m(2)). Here we show that UV-induced RPA-p34 hyperphosphorylation depends on expression of ATM, the product of the gene mutated in the human genetic disorder ataxia telangiectasia (A-T). UV-induced RPA-p34 hyperphosphorylation was not observed in A-T cells, but this response was restored by ATM expression. Furthermore, purified ATM kinase phosphorylates the p34 subunit of RPA complex in vitro at many of the same sites that are phosphorylated in vivo after UV radiation. Induction of this DNA damage response was also dependent on DNA replication; inhibition of DNA replication by aphidicolin prevented induction of RPA-p34 hyperphosphorylation by UV radiation. We postulate that this pathway is triggered by the accumulation of aberrant DNA replication intermediates, resulting from DNA replication fork blockage by UV photoproducts. Further, we suggest that RPA-p34 is hyperphosphorylated as a participant in the recombinational postreplication repair of these replication products. Successful resolution of these replication intermediates reduces the accumulation of chromosomal aberrations that would otherwise occur as a consequence of UV radiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.