ArticleClinical and experimental immunology2001
Age-related changes in membrane lipid composition, fluidity and respiratory burst in rat peritoneal neutrophils.
Article in Clinical and experimental immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 69 citations in OpenAlex.
- Immunosenescence: A systems-level overview of immune cell biology and strategies for improving vaccine responses.Experimental gerontology · 2019Pooled it
- Influence of phospholipid species on membrane fluidity: a meta-analysis for a novel phospholipid fluidity index.The Journal of membrane biology · 2011Pooled it
- Lipidomic profiling reveals age-dependent changes in plasma membrane lipids that affect neural stem cell aging.Science advances · 2026Article
- Review
- Immunosenescence, Inflammaging, and Frailty: Role of Myeloid Cells in Age-Related Diseases.Clinical reviews in allergy & immunology · 2023Review
- Review
- Membranes as the third genetic code.Molecular biology reports · 2020Review
- The Influence of Dietary Fatty Acids on Immune Responses.Nutrients · 2019Review
- The potential of non-myeloablative heterochronous autologous hematopoietic stem cell transplantation for extending a healthy life span.GeroScience · 2018Review
- Membrane Aging as the Real Culprit of Alzheimer's Disease: Modification of a Hypothesis.Neuroscience bulletin · 2018Review
- Immunosenescence and organ transplantation.Transplantation reviews (Orlando, Fla.) · 2013Review
- Biophysical characterization of membrane proteins in nanodiscs.Methods (San Diego, Calif.) · 2013Review
- Aging and immune function: molecular mechanisms to interventions.Antioxidants & redox signaling · 2011Review
- Aging of the innate immune system.Current opinion in immunology · 2010Review
- Human innate immunosenescence: causes and consequences for immunity in old age.Trends in immunology · 2009Review
- The association between angiotensin II-induced free radical generation and membrane fluidity in neutrophils of patients with metabolic syndrome.The Journal of membrane biology · 2006Article
- Cholesterol stimulates the cellular uptake of L-carnitine by the carnitine/organic cation transporter novel 2 (OCTN2).The Journal of biological chemistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The O2*(-) production has been studied in rat peritoneal neutrophils of different age (3, 12 and 24 months), in order to analyse whether the neutrophil respiratory burst is modified with increasing age. To stimulate NADPH oxidase, the enzyme responsible for the respiratory burst, two stimuli that act in different way have been used: phorbol myristate acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (N-FMLP). Production of O2*(-) decreased with age in neutrophils stimulated with N-FMLP (about 40%), but not in the stimulated with PMA. No difference in NADPH oxidase activity was found with age. The NADPH is supplied to the respiratory burst mainly by the pentose phosphate shunt. A progressive and significant decrease in the two most important enzymes of this route, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, was detected as a function of age; in spite of this reduction, the NADPH produced by cells from old animals seems not limiting for the O2*(-) production. The N-FMLP-induced decrease in the O2*(-) production may be related to the age-dependent increase in the membrane fluidity observed. A decline in the cholesterol/phospholipid ratio and a rise in the total polyunsaturated fatty acids content were found, that correlated well with the increase in the membrane fluidity. The decrease (50%) of phosphatidylinositols in the 24-month-old animals may be also related to the age-impairment in the respiratory burst found after stimulation with N-FMLP. These studies suggest that the age-related alterations in neutrophil may result in diminished neutrophil function and increased susceptibility to infection in the ageing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.