ArticleGut2001
Gastrointestinal cell proliferation and crypt fission are separate but complementary means of increasing tissue mass following infusion of epidermal growth factor in rats.
Article in Gut, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Current practice and future perspectives in the treatment of short bowel syndrome in children--a systematic review.Langenbeck's archives of surgery · 2012Pooled it
- Investigation of the clinical efficacy of mesenchymal stem cells in combination with monoclonal antibody therapy against feline panleukopenia.Stem cell research & therapy · 2026Article
- Intestinal Epithelial Axin1 Deficiency Protects Against Colitis via Altered Gut Microbiota.Engineering (Beijing, China) · 2024Article
- Mechanically enhanced biogenesis of gut spheroids with instability-driven morphomechanics.Nature communications · 2023Article
- The frequency of dysplastic branching crypts in colorectal polypoid tubular adenomas.International journal of experimental pathology · 2023Article
- A Fiber-Rich Diet and Radiation-Induced Injury in the Murine Intestinal Mucosa.International journal of molecular sciences · 2021Article
- Proliferation in the developing intestine is regulated by the endosomal protein Endotubin.Developmental biology · 2021Article
- Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids.Cell stem cell · 2021Article
- Systems Modeling to Quantify Safety Risks in Early Drug Development: Using Bifurcation Analysis and Agent-Based Modeling as Examples.The AAPS journal · 2021Review
- Epidermal growth factor receptor inhibitor-induced diarrhea: clinical incidence, toxicological mechanism, and management.Toxicology research · 2021Review
- Systems biology analysis identifies molecular determinants of chemotherapy-induced diarrhoea.Journal of molecular medicine (Berlin, Germany) · 2020Article
- Epidermal growth factor suppresses intestinal epithelial cell shedding through a MAPK-dependent pathway.Journal of cell science · 2017Article
- A Novel Role of Spred2 in the Colonic Epithelial Cell Homeostasis and Inflammation.Scientific reports · 2016Article
- Dietary Methyl Donor Depletion Suppresses Intestinal Adenoma Development.Cancer prevention research (Philadelphia, Pa.) · 2016Article
- Epidermal growth factor attenuates tubular necrosis following mercuric chloride damage by regeneration of indigenous, not bone marrow-derived cells.Journal of cellular and molecular medicine · 2015Article
- Development of the rat model of lapatinib-induced diarrhoea.Scientifica · 2014Review
- Assessment of the mode of action underlying development of rodent small intestinal tumors following oral exposure to hexavalent chromium and relevance to humans.Critical reviews in toxicology · 2013Review
- Diabetic lower extremity wounds: the rationale for growth factors-based infiltration treatment.International wound journal · 2011Review
- Musashi1 and hairy and enhancer of split 1 high expression cells derived from embryonic stem cells enhance the repair of small-intestinal injury in the mouse.Digestive diseases and sciences · 2011Article
- The intestinotrophic peptide, GLP-2, counteracts the gastrointestinal atrophy in mice induced by the epidermal growth factor receptor inhibitor, erlotinib, and cisplatin.Digestive diseases and sciences · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsEpidermal growth factor (EGF) is a potent mitogen for the gastrointestinal tract and also influences the number of new crypts formed by crypt fission. The time course of these events and possible linkage between these two complementary mechanisms is however poorly understood. We therefore examined the temporal relationship of proliferation and fission in rats treated with EGF.
methodsOsmotic minipumps were implanted subcutaneously into male Wistar rats to infuse EGF continuously (60 microg/rat/day) for periods of 1-14 days. Proliferation and crypt branching were quantified following vincristine induced metaphase arrest and morphometric assessment of microdissected tissue.
resultsIn the small intestine, EGF significantly increased epithelial cell proliferation and crypt and villus area after 24 hours of EGF, although maximal effects were only reached following six days of infusion. EGF also resulted in an approximate 30% reduction in crypt fission in the small bowel. In the colon, EGF caused a twofold increase in epithelial cell proliferation one day after infusion, from 15.3 (2.3) to 29.6 (3.5) metaphases per crypt (p<0.01). Maximal effects were seen in rats receiving EGF for seven days. For all time points, colonic crypt size increased in response to EGF. The amount of branching increased following one day of infusion with EGF (from 15.3 (1.9) to 32.4 (5.5)%; p<0.001) but was significantly lower (approximately 25% of control values) following longer periods of infusion. Crypt fission did not correlate with crypt area.
conclusionEGF has profound effects on cell proliferation and also altered crypt fission, with its actions on crypt fission most pronounced in the colon where it first increased and then decreased fission. EGF can thus be a potent stimulus for crypt fission during short term infusion and may reduce the number of branched crypts present in a resting or quiescent stage. Growth factors can alter cell mass by two separate but linked mechanisms, namely altered cell production and crypt fission.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.