ArticleGut2001
Inhibition of carbachol stimulated acid secretion by interleukin 1beta in rabbit parietal cells requires protein kinase C.
Article in Gut, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
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- To be or not to be: The host genetic factor and beyond in Helicobacter pylori mediated gastro-duodenal diseases.World journal of gastroenterology · 2015Review
- The role of obesity in oesophageal cancer development.Therapeutic advances in gastroenterology · 2014Review
- Intracellular calcium release and protein kinase C activation stimulate sonic hedgehog gene expression during gastric acid secretion.Gastroenterology · 2010Article
- TLR-independent induction of human monocyte IL-1 by phosphoglycolipids from thermophilic bacteria.Glycoconjugate journal · 2008Article
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- No relationship between IL-1B gene polymorphism and gastric acid secretion in younger healthy volunteers.World journal of gastroenterology · 2005Article
- Article
- Inhibition of small-conductance Cl- channels by the interleukin-1beta-stimulated production of superoxide in rabbit gastric parietal cells.The Journal of physiology · 2003Article
- Protein kinase C-alpha attenuates cholinergically stimulated gastric acid secretion of rabbit parietal cells.British journal of pharmacology · 2003Article
- Quantitative evaluation of inflammatory and immune responses in the early stages of chronic Helicobacter pylori infection.Infection and immunity · 2003Article
- Role of proinflammatory cytokines in the impaired lacrimation associated with autoimmune xerophthalmia.Investigative ophthalmology & visual science · 2002Article
- Effect of interlukin-1beta on proliferation of gastric epithelial cells in culture.BMC gastroenterology · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInterleukin 1beta (IL-1beta) is a potent inhibitor of gastric acid secretion. Regulatory actions at several levels have previously been demonstrated, including direct inhibition of parietal cell acid secretion. Although IL-1beta may activate several intracellular signalling pathways, the mechanisms responsible for inhibition of carbachol stimulated acid secretion have not been determined.
aimsTo investigate the roles of protein kinase C (PKC) and the sphingomyelinase signalling pathways in the regulation of acid secretion by IL-1beta.
methodsRabbit parietal cells were obtained by collagenase-EDTA digestion and centrifugal elutriation. Acid secretion stimulated by carbachol and A23187 (to mimic elevations in intracellular calcium) was assessed by 14C aminopyrine uptake in response to IL-1beta, PKC, and sphingomyelinase manipulation.
resultsIL-1beta inhibited carbachol and A23187 stimulated acid secretion in a dose dependent manner. The inhibitory actions were completely reversed by each of three different PKC inhibitors, staurosporine, H-7, and chelerythrine, as well as by PKC depletion with high dose phorbol ester pretreatment. IL-1beta did not downregulate parietal cell muscarinic receptor. IL-1beta significantly increased membrane PKC activity. Activation of the sphingomyelinase/ceramide pathway had no effect on basal or stimulated acid secretion. The inhibitory action of IL-1beta was independent of protein kinase A and protein kinase G activity.
conclusionsIL-1beta directly inhibits parietal cell carbachol stimulated acid secretion. This action occurs distal to muscarinic receptor activation and elevations in intracellular calcium and requires PKC.
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