ArticleGenes & development2001
Nitric oxide synthase is induced in sporulation of Physarum polycephalum.
Article in Genes & development, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Spermidine stimulates hypocrellin A biosynthesis through nitric oxide signaling in Shiraia sp. S9.Bioresources and bioprocessing · 2026Article
- Nitric Oxide in Fungi: Production and Function.Journal of fungi (Basel, Switzerland) · 2024Review
- Article
- Cell polarity signalling at the birth of multicellularity: What can we learn from the first animals.Frontiers in cell and developmental biology · 2022Review
- Studying Protista WBR and Repair Using Physarum polycephalum.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Role of PPAR Alpha in the Modulation of Innate Immunity.International journal of molecular sciences · 2021Review
- Regulatory Dynamics of Cell Differentiation Revealed by True Time Series From Multinucleate Single Cells.Frontiers in genetics · 2020Article
- An ancient role for nitric oxide in regulating the animal pelagobenthic life cycle: evidence from a marine sponge.Scientific reports · 2016Article
- Identification and functional analysis of endogenous nitric oxide in a filamentous fungus.Scientific reports · 2016Article
- The Physarum polycephalum Genome Reveals Extensive Use of Prokaryotic Two-Component and Metazoan-Type Tyrosine Kinase Signaling.Genome biology and evolution · 2015Article
- Evolutionary roots of arginase expression and regulation.Frontiers in immunology · 2014Review
- Article
- Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa).European journal of histochemistry : EJH · 2012Article
- Evaluation of endothelial cells differentiated from amniotic fluid-derived stem cells.Tissue engineering. Part A · 2012Article
- Parallel evolution of nitric oxide signaling: diversity of synthesis and memory pathways.Frontiers in bioscience (Landmark edition) · 2011Review
- Sleep deprivation triggers inducible nitric oxide-dependent nitric oxide production in wake-active basal forebrain neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Cyclic GMP as a second messenger in the nitric oxide-mediated conidiation of the mycoparasite Coniothyrium minitans.Applied and environmental microbiology · 2010Article
- Transcriptomic changes arising during light-induced sporulation in Physarum polycephalum.BMC genomics · 2010Article
- Nitric oxide as a signaling molecule in the fission yeast Schizosaccharomyces pombe.Protoplasma · 2009Article
- Physarum nitric oxide synthases: genomic structures and enzymology of recombinant proteins.The Biochemical journal · 2009Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The myxomycete Physarum polycephalum expresses a calcium-independent nitric oxide (NO) synthase (NOS) resembling the inducible NOS isoenzyme in mammals. We have now cloned and sequenced this, the first nonanimal NOS to be identified, showing that it shares < 39% amino acid identity with known NOSs but contains conserved binding motifs for all NOS cofactors. It lacks the sequence insert responsible for calcium dependence in the calcium-dependent NOS isoenzymes. NOS expression was strongly up-regulated in Physarum macroplasmodia during the 5-day starvation period needed to induce sporulation competence. Induction of both NOS and sporulation competence were inhibited by glucose, a growth signal and known repressor of sporulation, and by L-N6-(1-iminoethyl)-lysine (NIL), an inhibitor of inducible NOS. Sporulation, which is triggered after the starvation period by light exposure, was also prevented by 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ), an inhibitor of NO-sensitive guanylate cyclase. In addition, also expression of lig1, a sporulation-specific gene, was strongly attenuated by NIL or ODQ. 8-Bromo-cGMP, added 2 h before the light exposure, restored the capacity of NIL-treated macroplasmodia to express lig1 and to sporulate. This indicates that the second messenger used for NO signaling in sporulation of Physarum is cGMP and links this signaling pathway to expression of lig1.
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