ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Direct visualization of the movement of the monomeric axonal transport motor UNC-104 along neuronal processes in living Caenorhabditis elegans.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 106 citations in OpenAlex.
- Article
- KLP-7/Kinesin-13 orchestrates axon-dendrite checkpoints for polarized trafficking in neurons.Molecular biology of the cell · 2024Article
- F-box protein FBXB-65 regulates anterograde transport of the kinesin-3 motor UNC-104 through a PTM near its cargo-binding PH domain.Journal of cell science · 2024Article
- Analyzing Photoactivation with Diffusion Models to Study Transport in the Endoplasmic Reticulum Network.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Kymolyzer, a Semi-Autonomous Kymography Tool to Analyze Intracellular Motility.Current protocols in cell biology · 2020Article
- Computational design and interpretation of single-RNA translation experiments.PLoS computational biology · 2019Article
- The Kinesin-3 motor, KLP-4, mediates axonal organization and cholinergic signaling inFASEB bioAdvances · 2019Article
- Physical parameters describing neuronal cargo transport by kinesin UNC-104.Biophysical reviews · 2019Review
- Article
- Fluorescence microscopy applied to intracellular transport by microtubule motors.Journal of biosciences · 2018Review
- Coupling of Rigor Mortis and Intestinal Necrosis during C. elegans Organismal Death.Cell reports · 2018Article
- Non-neuronal cell outgrowth inWorm · 2017Review
- A fast and scalable kymograph alignment algorithm for nanochannel-based optical DNA mappings.PloS one · 2015Article
- Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84.Journal of neuroscience research · 2014Article
- Dimerization of mammalian kinesin-3 motors results in superprocessive motion.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Recycling of kinesin-1 motors by diffusion after transport.PloS one · 2013Article
- The kinesin-3 family motor KLP-4 regulates anterograde trafficking of GLR-1 glutamate receptors in the ventral nerve cord of Caenorhabditis elegans.Molecular biology of the cell · 2012Article
- Cyclin-dependent kinase 5 regulates the polarized trafficking of neuropeptide-containing dense-core vesicles in Caenorhabditis elegans motor neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- UNC-33 (CRMP) and ankyrin organize microtubules and localize kinesin to polarize axon-dendrite sorting.Nature neuroscience · 2011Article
- Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The formation and function of axons depends on the microtubule-based transport of cellular components from their sites of synthesis in the neuronal cell body to their sites of utilization at the axon terminus. To directly visualize this axonal transport in a living organism, we constructed transgenic lines of Caenorhabditis elegans that express green fluorescent protein fused to the monomeric synaptic vesicle transport motor, UNC-104. This UNC-104:: GFP construct rescued the Unc-104 mutant phenotype and was expressed throughout the nervous system. Using time-lapse confocal fluorescence microscopy, we were able to visualize fluorescent motor proteins moving in both directions along neuronal processes, some of which were identified definitely as axons and others as dendrites. Using kymograph analysis, we followed the movement of >900 particles. Most of them moved in one direction, but not necessarily at the same velocity. Ten percent of the observed particles reversed direction of movement during the period of observation, and 10% exhibited periods of movement interspersed with pauses. During episodes of persistent movement, particles moved at an average velocity of 1.02 microm/sec, which is close to the in vitro velocity of microtubule gliding driven by purified monomeric kinesin at high motor density. To our knowledge, this is the first direct visualization and analysis of the movement of specifically labeled microtubule motor proteins along axons in vivo.
Indexed as
Identifiers
11356862PMC6762686W1509322093What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.