ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Involvement of spinal protein kinase Cgamma in the attenuation of opioid mu-receptor-mediated G-protein activation after chronic intrathecal administration of [D-Ala2,N-MePhe4,Gly-Ol(5)]enkephalin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 66 citations in OpenAlex.
- Proteomic analysis of synaptic protein turnover in the anterior cingulate cortex after nerve injury.Molecular brain · 2020Article
- Antinociceptive Activity of Methanolic Extract ofPain research & management · 2018Article
- cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice.Molecular medicine reports · 2017Article
- Narcolepsy-like sleep disturbance in orexin knockout mice are normalized by the 5-HT1A receptor agonist 8-OH-DPAT.Psychopharmacology · 2016Article
- Pain after discontinuation of morphine treatment is associated with synaptic increase of GluA4-containing AMPAR in the dorsal horn of the spinal cord.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2013Article
- Differential activation of the μ-opioid receptor by oxycodone and morphine in pain-related brain regions in a bone cancer pain model.British journal of pharmacology · 2013Article
- Characterization of basal and morphine-induced uridine release in the striatum: an in vivo microdialysis study in mice.Neurochemical research · 2013Article
- Involvement of NOX1/NADPH oxidase in morphine-induced analgesia and tolerance.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Change in microRNAs associated with neuronal adaptive responses in the nucleus accumbens under neuropathic pain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Morphine-induced receptor endocytosis in a novel knockin mouse reduces tolerance and dependence.Current biology : CB · 2008Article
- Resistance to morphine analgesic tolerance in rats with deleted transient receptor potential vanilloid type 1-expressing sensory neurons.Neuroscience · 2007Article
- Opioids: a review.Current pain and headache reports · 2003Review
- Chronic morphine induces downregulation of spinal glutamate transporters: implications in morphine tolerance and abnormal pain sensitivity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Neuronal apoptosis associated with morphine tolerance: evidence for an opioid-induced neurotoxic mechanism.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- The neurobiology of opiate tolerance, dependence and sensitization: mechanisms of NMDA receptor-dependent synaptic plasticity.Neurotoxicity research · 2002Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The present study was designed to investigate the role of a protein kinase C (PKC) isoform in the uncoupling of the mu-opioid receptor from G-proteins after repeated intrathecal injection of a selective mu-receptor agonist, [D-Ala(2),N-MePhe(4),Gly-ol(5)]enkephalin (DAMGO), in the spinal cord of mice. The activation of G-proteins by opioids was measured by monitoring the guanosine-5'-o-(3-[(35)S]thio)triphosphate ([(35)S]GTPgammaS) binding. Mice were injected intrathecally with saline or DAMGO once a day for 1-7 d. At 24 hr after every injection the spinal cord membranes were prepared for the assay. The enhanced [(35)S]GTPgammaS binding by mu-agonists DAMGO, endomorphin-1, or endomorphin-2 was attenuated clearly in spinal cord membranes obtained from mice that were treated intrathecally with DAMGO for 5 and 7 d, but not for 1 or 3 d. By contrast, no change in levels of [(35)S]GTPgammaS binding induced by the delta-receptor agonist SNC-80 or kappa-receptor agonist U-50,488H was noted in membranes obtained from mice that were treated with DAMGO. Concomitant intrathecal administration of a specific PKC inhibitor Ro-32-0432 with DAMGO blocked the attenuation of DAMGO-induced G-protein activation that was caused by chronic DAMGO treatment. Western blotting analysis showed that chronic DAMGO treatment increased the levels of PKCgamma, but not PKCalpha, PKCbetaI, and PKCbetaII isoforms, in spinal cord membranes. Furthermore, mice lacking PKCgamma failed to exhibit the desensitization of the DAMGO-stimulated [(35)S]GTPgammaS binding after repeated DAMGO injection. These findings indicate that repeated intrathecal administration of DAMGO may activate the PKCgamma isoform and in turn cause a desensitization of mu-receptor-mediated G-protein activation in the mouse spinal cord.
Indexed as
Identifiers
11356858PMC6762716W1896872049What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.