ArticleProceedings of the National Academy of Sciences of the United States of America2001
Sense- and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 62 citations in OpenAlex.
- Radical Oxygen Species, Oxidized Low-Density Lipoproteins, and Lectin-like Oxidized Low-Density Lipoprotein Receptor 1: A Vicious Circle in Atherosclerotic Process.Antioxidants (Basel, Switzerland) · 2024Review
- Coordinated Action of RTBV and RTSV Proteins Suppress Host RNA Silencing Machinery.Microorganisms · 2022Article
- Differential Contribution of RNA Interference Components in Response to Distinct Fusarium graminearum Virus Infections.Journal of virology · 2018Article
- An intron sense suppression construct targeting soybean FAD2-1 requires a double-stranded RNA-producing inverted repeat T-DNA insert.Plant physiology · 2010Article
- RNA silencing mediated by direct repeats in maize: a potential tool for functional genomics.Molecular biotechnology · 2009Article
- Active RNA silencing at low temperature indicates distinct pathways for antisense-mediated gene-silencing in potato.Plant molecular biology · 2005Article
- Gene silencing studies in the gymnosperm species Pinus radiata.Plant cell reports · 2005Article
- Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato.Plant cell reports · 2005Article
- A systemic small RNA signaling system in plants.The Plant cell · 2004Article
- Characteristics of RNA silencing in plants: similarities and differences across kingdoms.Plant molecular biology · 2004Review
- RNA interference: biology, mechanism, and applications.Microbiology and molecular biology reviews : MMBR · 2003Review
- Resistance of human hepatitis delta virus RNAs to dicer activity.Journal of virology · 2003Article
- Deletion derivatives of the MuDR regulatory transposon of maize encode antisense transcripts but are not dominant-negative regulators of mutator activities.The Plant cell · 2003Article
- RNA interference: antiviral weapon and beyond.World journal of gastroenterology · 2003Review
- Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation.The EMBO journal · 2003Article
- The coat protein of turnip crinkle virus suppresses posttranscriptional gene silencing at an early initiation step.Journal of virology · 2003Article
- High molecular weight RNAs and small interfering RNAs induce systemic posttranscriptional gene silencing in plants.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A viral protein inhibits the long range signaling activity of the gene silencing signal.The EMBO journal · 2002Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antisense-mediated gene silencing (ASGS) and posttranscriptional gene silencing (PTGS) with sense transgenes markedly reduce the steady-state mRNA levels of endogenous genes similar in transcribed sequence. RNase protection assays established that silencing in tobacco plants transformed with plant-defense-related class I sense and antisense chitinase (CHN) transgenes is at the posttranscriptional level. Infection of tobacco plants with cucumber mosaic virus strain FN and a necrotizing strain of potato virus Y, but not with potato virus X, effectively suppressed PTGS and ASGS of both the transgenes and homologous endogenes. This suggests that ASGS and PTGS share components associated with initiation and maintenance of the silent state. Small, ca. 25-nt RNAs (smRNA) of both polarities were associated with PTGS and ASGS in CHN transformants as reported for PTGS in other transgenic plants and for RNA interference in Drosophila. Similar results were obtained with an antisense class I beta-1,3-glucanase transformant showing that viral suppression and smRNAs are a more general feature of ASGS. Several current models hold that diverse signals lead to production of double-stranded RNAs, which are processed to smRNAs that then trigger PTGS. Our results provide direct evidence for mechanistic links between ASGS and PTGS and suggest that ASGS could join a common PTGS pathway at the double-stranded RNA step.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.