ArticleAntimicrobial agents and chemotherapy2001
Genomic profiling of the response of Candida albicans to itraconazole treatment using a DNA microarray.
Article in Antimicrobial agents and chemotherapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
80 citing papers in PubMed, 210 citations in OpenAlex.
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- A Proteomic Landscape of Candida albicans in the Stepwise Evolution to Fluconazole Resistance.Antimicrobial agents and chemotherapy · 2022Article
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- Study on the anti-biofilm mechanism of 1,8-cineole againstFrontiers in pharmacology · 2022Article
- Ketoconazole resistant Candida albicans is sensitive to a wireless electroceutical wound care dressing.Bioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- Two Functionally Redundant FK506-Binding Proteins Regulate Multidrug Resistance Gene Expression and Govern Azole Antifungal Resistance.Antimicrobial agents and chemotherapy · 2021Article
- In Vitro Antifungal Activity and Mechanism of AgMolecules (Basel, Switzerland) · 2020Article
- Drug-Resistant Fungi: An Emerging Challenge Threatening Our Limited Antifungal Armamentarium.Antibiotics (Basel, Switzerland) · 2020Review
- Transcriptional response ofMicrobiology (Reading, England) · 2020Article
- Virulence and biofilms as promising targets in developing antipathogenic drugs against candidiasis.Future science OA · 2020Review
- Recent trends in molecular diagnostics of yeast infections: from PCR to NGS.FEMS microbiology reviews · 2019Review
- Anticandidal Potential of Stem Bark Extract fromMolecules (Basel, Switzerland) · 2019Article
- Eucalyptal D Enhances the Antifungal Effect of Fluconazole on Fluconazole-ResistantFrontiers in cellular and infection microbiology · 2019Article
- In Vitro Anticandidal Activity and Mechanism of a Polyoxovanadate Functionalized by Zn-Fluconazole Complexes.Molecules (Basel, Switzerland) · 2018Article
- In vitro and in vivo antifungal activities and mechanism of heteropolytungstates against Candida species.Scientific reports · 2017Article
- Mitochondrial Cochaperone Mge1 Is Involved in Regulating Susceptibility to Fluconazole inmBio · 2017Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of genome-wide expression profiling to determine how drugs achieve their therapeutic effect has provided the pharmaceutical industry with an exciting new tool for drug mode-of-action studies. We used DNA chip technology to study cellular responses to perturbations of ergosterol biosynthesis caused by the broad-spectrum antifungal agent itraconazole. Simultaneous examination of over 6,600 Candida albicans gene transcript levels, representing the entire genome, upon treatment of cells with 10 microM itraconazole revealed that 296 genes were responsive. For 116 genes transcript levels were decreased at least 2.5-fold, while for 180 transcript levels were similarly increased. A global upregulation of ERG genes in response to azole treatment was observed. ERG11 and ERG5 were found to be upregulated approximately 12-fold. In addition, a significant upregulation was observed for ERG6, ERG1, ERG3, ERG4, ERG10, ERG9, ERG26, ERG25, ERG2, IDII, HMGS, NCP1, and FEN2, all of which are genes known to be involved in ergosterol biosynthesis. The effects of itraconazole on a wide variety of known metabolic processes are discussed. As over 140 proteins with unknown function were responsive to itraconazole, our analysis might provide-in combination with phenotypic data-first hints of their potential function. The present report is the first to describe the application of DNA chip technology to study the response of a major human fungal pathogen to drug treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.