ArticleNucleic acids research2001
Characterization of oligodeoxyribonucleotide synthesis on glass plates.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Fabrication of microcolumn arrays for high-throughput oligonucleotide synthesis using 3D printing.Scientific reports · 2025Article
- Recent progress in DNA data storage based on high-throughput DNA synthesis.Biomedical engineering letters · 2024Review
- Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates.Analytical chemistry · 2024Article
- Dipodal Silanes Greatly Stabilize Glass Surface Functionalization for DNA Microarray Synthesis and High-Throughput Biological Assays.Analytical chemistry · 2023Article
- DNA synthesis technologies to close the gene writing gap.Nature reviews. Chemistry · 2023Review
- Chemical and photochemical error rates in light-directed synthesis of complex DNA libraries.Nucleic acids research · 2021Article
- Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography.Chemical communications (Cambridge, England) · 2014Article
- 'Protected DNA Probes' capable of strong hybridization without removal of base protecting groups.Nucleic acids research · 2008Article
- Mutations in four regulatory genes have interrelated effects on heterocyst maturation in Anabaena sp. strain PCC 7120.Journal of bacteriology · 2006Article
- Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis.Nucleic acids research · 2004Article
- Effects of stray light on the fidelity of photodirected oligonucleotide array synthesis.Nucleic acids research · 2002Article
- An assessment of Motorola CodeLink microarray performance for gene expression profiling applications.Nucleic acids research · 2002Article
- A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids.Nucleic acids research · 2001Article
- The evolving art of creating genetic diversity: From directed evolution to synthetic biology.Biotechnology advancesReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Achieving high fidelity chemical synthesis on glass plates has become increasingly important, since glass plates are substrates widely used for miniaturized chemical and biochemical reactions and analyses. DNA chips can be directly prepared by synthesizing oligonucleotides on glass plates, but the characterization of these micro-syntheses has been limited by the sub-picomolar amount of material available. Most DNA chip syntheses have been assayed using in situ coupling of fluorescent molecules to the 5'-OH of the synthesized oligonucleotides. We herein report a systematic investigation of oligonucleotide synthesis on glass plates with the reactions carried out in an automated DNA synthesizer using standard phosphoramidite chemistry. The analyses were performed using (32)P gel electrophoresis of the oligonucleotides cleaved from glass plates to provide product distribution profiles according to chain length of oligonucleotides. 5'-Methoxythymidine was used as the chain terminator, which permits assay of coupling reaction yields as a function of chain length growth. The results of this work reveal that a major cause of lower fidelity synthesis on glass plates is particularly inefficient reactions of the various reagents with functional groups close to glass plate surfaces. These problems cannot be detected by previous in situ fluorescence assays. The identification of this origin of low fidelity synthesis on glass plates should help to achieve improved synthesis for high quality oligonucleotide microarrays.
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