ArticleThe Journal of cell biology2001
Role of phosphatidylinositol 3' kinase and a downstream pleckstrin homology domain-containing protein in controlling chemotaxis in dictyostelium.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers, 1 of them a synthesis that pooled it.
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Who cites it
106 citing papers in PubMed, 1 synthesis or guideline pooled it, 227 citations in OpenAlex.
- A meta-analysis indicates that the regulation of cell motility is a non-intrinsic function of chemoattractant receptors that is governed independently of directional sensing.Frontiers in immunology · 2022Pooled it
- Mechanism of activation of an ancestral TEC kinase by PIPNpj structural biology · 2026Article
- Ancestral TALE homeobox protein transcription factor regulates actin dynamics and cellular activities of protozoan parasite Entamoeba invadens.Molecular microbiology · 2024Article
- Dynamics of Actin Cytoskeleton and Their Signaling Pathways during Cellular Wound Repair.Cells · 2022Article
- Gradients of PI(4,5)PFrontiers in cell and developmental biology · 2022Article
- Leep1 interacts with PIP3 and the Scar/WAVE complex to regulate cell migration and macropinocytosis.The Journal of cell biology · 2021Article
- From environmental sensing to developmental control: cognitive evolution in dictyostelid social amoebas.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Ras, PI3K and mTORC2 - three's a crowd?Journal of cell science · 2020Review
- Single-molecule imaging of PI(4,5)PCommunications biology · 2020Article
- CD2AP links actin to PI3 kinase activity to extend epithelial cell height and constrain cell area.The Journal of cell biology · 2020Article
- Physical constraints on accuracy and persistence during breast cancer cell chemotaxis.PLoS computational biology · 2019Article
- A calcium-dependent phospholipase A2 (cPLA2) expression is regulated by MIG-6 during endometrial tumorigenesis.Biochemical and biophysical research communications · 2019Article
- Excitable dynamics of Ras triggers spontaneous symmetry breaking of PIP3 signaling in motile cells.Journal of cell science · 2019Article
- Collective cell migration ofCommunications biology · 2019Article
- GRAMD1B regulates cell migration in breast cancer cells through JAK/STAT and Akt signaling.Scientific reports · 2018Article
- GPCR-controlled membrane recruitment of negative regulator C2GAP1 locally inhibits Ras signaling for adaptation and long-range chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Function and Regulation of Heterotrimeric G Proteins during Chemotaxis.International journal of molecular sciences · 2016Review
- Article
- Single-molecule tracking of small GTPase Rac1 uncovers spatial regulation of membrane translocation and mechanism for polarized signaling.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Microtubule-Mediated Inositol Lipid Signaling Plays Critical Roles in Regulation of Blebbing.PloS one · 2015Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that cells lacking two Dictyostelium class I phosphatidylinositol (PI) 3' kinases (PI3K and pi3k1/2-null cells) or wild-type cells treated with the PI3K inhibitor LY294002 are unable to properly polarize, are very defective in the temporal, spatial, and quantitative regulation of chemoattractant-mediated filamentous (F)-actin polymerization, and chemotax very slowly. PI3K is thought to produce membrane lipid-binding sites for localization of PH domain-containing proteins. We demonstrate that in response to chemoattractants three PH domain-containing proteins do not localize to the leading edge in pi3k1/2-null cells, and the translocation is blocked in wild-type cells by LY294002. Cells lacking one of these proteins, phdA-null cells, exhibit defects in the level and kinetics of actin polymerization at the leading edge and have chemotaxis phenotypes that are distinct from those described previously for protein kinase B (PKB) (pkbA)-null cells. Phenotypes of PhdA-dominant interfering mutations suggest that PhdA is an adaptor protein that regulates F-actin localization in response to chemoattractants and links PI3K to the control of F-actin polymerization at the leading edge during pseudopod formation. We suggest that PKB and PhdA lie downstream from PI3K and control different downstream effector pathways that are essential for proper chemotaxis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.