ArticlePlant molecular biology2001
Plant mitochondria contain proteolytic and regulatory subunits of the ATP-dependent Clp protease.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 74 citations in OpenAlex.
- Elucidating the Molecular Impact of DA29-Induced ClpP Chemoactivation on Mitochondrial Function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Effects of Independent and Combined Water-Deficit and High-Nitrogen Treatments on Flag Leaf Proteomes during Wheat Grain Development.International journal of molecular sciences · 2020Article
- The Chloroplast Envelope Protease FTSH11 - Interaction With CPN60 and Identification of Potential Substrates.Frontiers in plant science · 2019Article
- The Plastid and Mitochondrial Peptidase Network inThe Plant cell · 2017Review
- Genetic diagnosis of Mendelian disorders via RNA sequencing.Nature communications · 2017Article
- YeATS - a tool suite for analyzing RNA-seq derived transcriptome identifies a highly transcribed putative extensin in heartwood/sapwood transition zone in black walnut.F1000Research · 2015Article
- Structural and biochemical analyses of the eukaryotic heat shock locus V (HslV) from Trypanosoma brucei.The Journal of biological chemistry · 2013Article
- Defining the protein complex proteome of plant mitochondria.Plant physiology · 2011Article
- Subunit stoichiometry, evolution, and functional implications of an asymmetric plant plastid ClpP/R protease complex in Arabidopsis.The Plant cell · 2011Article
- Genome-wide analysis of rice ClpB/HSP100, ClpC and ClpD genes.BMC genomics · 2010Article
- Atypical caseinolytic protease homolog from Plasmodium falciparum possesses unusual substrate preference and a functional nuclear localization signal.Parasitology research · 2009Article
- Analysis of molecular markers in three different tomato cultivars exposed to ozone stress.Plant cell reports · 2008Article
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- An Arabidopsis thaliana virescent mutant reveals a role for ClpR1 in plastid development.Plant molecular biology · 2007Article
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- Inactivation of the clpC1 gene encoding a chloroplast Hsp100 molecular chaperone causes growth retardation, leaf chlorosis, lower photosynthetic activity, and a specific reduction in photosystem content.Plant physiology · 2004Article
- Expression in multigene families. Analysis of chloroplast and mitochondrial proteases.Plant physiology · 2004Article
- The Chlamydomonas genome reveals its secrets: chaperone genes and the potential roles of their gene products in the chloroplast.Photosynthesis research · 2004Article
- Gene expression during anthesis and senescence in Iris flowers.Plant molecular biology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proteolytic machinery of plant organelles is largely unknown, although indications so far point to several proteases of bacterial origin. In this study an Arabidopsis thaliana cDNA was isolated that encodes a homologue of bacterial ClpX, a molecular chaperone and regulatory subunit of the ATP-dependent, serine-type Clp protease. Computer analysis of the predicted plant ClpX revealed a putative mitochondrial transit peptide at the N-terminus, as well as overall sequence similarity to other eukaryotic ClpX homologues. Specific polyclonal antibodies were made to the Arabidopsis ClpX protein and used to confirm its localization in plant mitochondria. In addition to ClpX, a ClpP protein located in mitochondria was also identified from the numerous ClpP isomers in Arabidopsis. Localization of this nuclear-encoded protein, termed ClpP2, was determined first by its close sequence similarity to mitochondrial ClpP human, and later experimentally using ClpP2-specific antibodies with isolated plant organellar fractions. In Arabidopsis, transcripts for both clpX and clpP2 genes were detected in various tissues and under different growth conditions, with no significant variation in mRNA level (i.e. 2-fold) for each gene between samples. Using beta-casein as a substrate, plant mitochondria were found to possess an ATP-stimulated, serine-type proteolytic activity that could be strongly inhibited by antibodies specific for ClpX or ClpP2, suggesting an active ClpXP protease. The recent discovery of homologous mitochondrial ClpX and ClpP proteins in mammals suggests that this type of protease may be common to multicellular eukaryotes.
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