ArticlePlant molecular biology2001
Non-photosynthetic 'malic enzyme' from maize: a constituvely expressed enzyme that responds to plant defence inducers.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
- A milestone in C4 carbon concentration mechanism evolution: structural remodeling of NADP-malic enzyme in Poaceae.Molecular biology and evolution · 2026Article
- Identification and Abiotic Stress Expression Profiling of Malic Enzyme-Associated Genes in Maize (Plants (Basel, Switzerland) · 2025Article
- Article
- Independent Recruitment of Duplicated β-Subunit-Coding NAD-ME Genes Aided the Evolution of C4 Photosynthesis in Cleomaceae.Frontiers in plant science · 2020Article
- Chimeric Structure of Plant Malic Enzyme Family: Different Evolutionary Scenarios for NAD- and NADP-Dependent Isoforms.Frontiers in plant science · 2018Article
- Association of variation in the sugarcane transcriptome with sugar content.BMC genomics · 2017Article
- Genome-wide identification, classification, and analysis of NADP-ME family members from 12 crucifer species.Molecular genetics and genomics : MGG · 2016Article
- The Roles of Organic Acids in C4 Photosynthesis.Frontiers in plant science · 2016Review
- Partially resistant Cucurbita pepo showed late onset of the Zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar.Frontiers in plant science · 2015Article
- Kinetics and functional diversity among the five members of the NADP-malic enzyme family from Zea mays, a C4 species.Photosynthesis research · 2013Article
- Article
- Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana.The Journal of biological chemistry · 2010Article
- Article
- Effect of Potato virus Y on the NADP-malic enzyme from Nicotiana tabacum L.: mRNA, expressed protein and activity.International journal of molecular sciences · 2009Article
- Evolutionary insights on C4 photosynthetic subtypes in grasses from genomics and phylogenetics.Genome biology and evolution · 2009Article
- Comparative genomic analysis of C4 photosynthetic pathway evolution in grasses.Genome biology · 2009Article
- Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots.Plant molecular biology · 2008Article
- Arabidopsis NAD-malic enzyme functions as a homodimer and heterodimer and has a major impact on nocturnal metabolism.Plant physiology · 2008Article
- Alteration of organic acid metabolism in Arabidopsis overexpressing the maize C4 NADP-malic enzyme causes accelerated senescence during extended darkness.Plant physiology · 2007Article
- Characterization of the NADP malic enzyme gene family in the facultative, single-cell C4 monocot Hydrilla verticillata.Photosynthesis research · 2007Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform present in maize roots differs from the photosynthetic enzyme implicated in the C4 cycle, but is similar, or identical, to the enzyme previously characterized from etiolated maize leaves (Maurino, Drincovich and Andreo, Biochem. Mol. Biol. Int. 38 (1996) 239-250). A full-length ORF encoding a plastidic NADP-ME (almost identical to the maize root NADP-ME, GenBank accession number U39958) was cloned from a root cDNA library as well as isolated by reverse transcription (RT)-PCR using green leaves mRNA as template. These results indicate that root NADP-ME does not constitute a root-specific isoform, but represents a protein with a constitutive pattern of expression in plastids of the C4 plant maize. The amount of NADP-ME measured by activity, western and northern blot was modified when different stress conditions (including treatments with cellulase, fungal elicitors, jasmonate and hypoxic treatment) were applied to maize roots, indicating that the enzyme from maize roots is under transcriptional or post-transcriptional regulation by effectors related to plant defence responses. It is deduced that the induction of housekeeping genes, like non-photosynthetic NADP-ME, whose constitutive role may be the provision of reductive power in non-photosynthetic plastids, is likely to accompany the defence response.
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