ArticlePlant molecular biology2001
Transient GFP expression in Nicotiana plumbaginifolia suspension cells: the role of gene silencing, cell death and T-DNA loss.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 25 citations in OpenAlex.
- Single Molecule Imaging of T-DNA Intermediates FollowingInternational journal of molecular sciences · 2019Article
- Genetic variation assessment of stacked-trait transgenic maize via conventional breeding.BMC plant biology · 2019Article
- Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties.Environmental sciences Europe · 2016Article
- Effect of stacking insecticidal cry and herbicide tolerance epsps transgenes on transgenic maize proteome.BMC plant biology · 2014Article
- Enhanced transgene expression in sugarcane by co-expression of virus-encoded RNA silencing suppressors.PloS one · 2013Article
- Quantitative evaluation of six different viral suppressors of silencing using image analysis of transient GFP expression.Plant cell reports · 2009Article
- Quantification and extension of transient GFP expression by the co-introduction of a suppressor of silencing.Transgenic research · 2008Article
- Stimulation of the cell cycle and maize transformation by disruption of the plant retinoblastoma pathway.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transient nature of T-DNA expression was studied with a gfp reporter gene transferred to Nicotiana plumbaginifolia suspension cells from Agrobacterium tumefaciens. Individual GFP-expressing protoplasts were isolated after 4 days' co-cultivation. The protoplasts were cultured without selection and 4 weeks later the surviving proto-calluses were again screened for GFP expression. Of the proto-calluses initially expressing GFP, 50% had lost detectable GFP activity during the first 4 weeks of culture. Multiple T-DNA copies of the gfp gene were detected in 10 of 17 proto-calluses lacking visible GFP activity. The remaining 7 cell lines contained no gfp sequences. Our results confirm that transiently expressed T-DNAs can be lost during growth of somatic cells and demonstrate that transiently expressing cells frequently integrate multiple T-DNAs that become silenced. In cells competent for DNA uptake, cell death and gene silencing were more important barriers to the recovery of stably expressing transformants than lack of T-DNA integration.
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