ArticlePlant physiology2001
The internal propeptide of the ricin precursor carries a sequence-specific determinant for vacuolar sorting.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 98 citations in OpenAlex.
- Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin.Biotechnology and bioengineering · 2019Article
- Unique Motifs and Length of Hairpin in Oleosin Target the Cytosolic Side of Endoplasmic Reticulum and Budding Lipid Droplet.Plant physiology · 2017Article
- Ribosome-Inactivating Proteins from Plants: A Historical Overview.Molecules (Basel, Switzerland) · 2016Review
- Glycosylphosphatidylinositol (GPI) Modification Serves as a Primary Plasmodesmal Sorting Signal.Plant physiology · 2016Article
- Article
- Multiple internal sorting determinants can contribute to the trafficking of cruciferin to protein storage vacuoles.Plant molecular biology · 2015Article
- The C-terminal propeptide of a plant defensin confers cytoprotective and subcellular targeting functions.BMC plant biology · 2014Article
- Article
- Analysis of castor bean ribosome-inactivating proteins and their gene expression during seed development.Genetics and molecular biology · 2013Article
- Mechanisms and concepts paving the way towards a complete transport cycle of plant vacuolar sorting receptors.The Plant cell · 2012Review
- Secretory pathway research: the more experimental systems the better.The Plant cell · 2012Review
- Ricin trafficking in plant and mammalian cells.Toxins · 2011Review
- Review
- Ricin B chain targeted to the endoplasmic reticulum of tobacco protoplasts is degraded by a CDC48- and vacuole-independent mechanism.The Journal of biological chemistry · 2008Article
- Article
- Sorting and anterograde trafficking at the Golgi apparatus.Plant physiology · 2008Article
- Pollination in Nicotiana alata stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue.Journal of experimental botany · 2008Article
- Fluorescent reporter proteins for the tonoplast and the vacuolar lumen identify a single vacuolar compartment in Arabidopsis cells.Plant physiology · 2007Article
- Arabidopsis vacuolar sorting mutants (green fluorescent seed) can be identified efficiently by secretion of vacuole-targeted green fluorescent protein in their seeds.The Plant cell · 2007Article
- The C-terminal region of alpha' subunit of soybean beta-conglycinin contains two types of vacuolar sorting determinants.Plant molecular biology · 2006Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ricin is a heterodimeric toxin that accumulates in the storage vacuoles of castor bean (Ricinus communis) endosperm. Proricin is synthesized as a single polypeptide precursor comprising the catalytic A chain and the Gal-binding B chain joined by a 12-amino acid linker propeptide. Upon arrival in the vacuole, the linker is removed. Here, we replicate these events in transfected tobacco (Nicotiana tabacum) leaf protoplasts. We show that the internal linker propeptide is responsible for vacuolar sorting and is sufficient to redirect the ricin heterodimer to the vacuole when fused to the A or the B chain. This internal peptide can also target two different secretory protein reporters to the vacuole. Moreover, mutation of the isoleucine residue within an NPIR-like motif of the propeptide affects vacuolar sorting in proricin and in the reconstituted A-B heterodimer. This is the first reported example of a sequence-specific vacuolar sorting signal located within an internal propeptide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.