ArticlePlant physiology2001
Constitutive overexpression of cytosolic glutamine synthetase (GS1) gene in transgenic alfalfa demonstrates that GS1 may be regulated at the level of RNA stability and protein turnover.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.
- Genetically modified crops are superior in their nitrogen use efficiency-A meta-analysis of three major cereals.Scientific reports · 2020Pooled it
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- The Role of Glutamine Synthetase (GS) and Glutamate Synthase (GOGAT) in the Improvement of Nitrogen Use Efficiency in Cereals.Biomolecules · 2023Review
- Physiological and proteomics responses of nitrogen assimilation and glutamine/glutamine family of amino acids metabolism in mulberry (Plant signaling & behavior · 2020Article
- Dynamic changes impact the plum pox virus population structure during leaf and bud development.Virology · 2020Article
- Nitrogen Regulating the Expression and Localization of Four Glutamine Synthetase Isoforms in Wheat (International journal of molecular sciences · 2020Article
- Article
- The Effect of PoplarFrontiers in plant science · 2018Article
- Article
- Genome-wide survey of the soybean GATA transcription factor gene family and expression analysis under low nitrogen stress.PloS one · 2015Article
- Article
- Glutamine synthetase in Medicago truncatula, unveiling new secrets of a very old enzyme.Frontiers in plant science · 2015Review
- Characterization of a glutamine synthetase gene DvGS1 from Dunaliella viridis and investigation of the impact on expression of DvGS1 in transgenic Arabidopsis thaliana.Molecular biology reports · 2014Article
- The 5' untranslated region of the soybean cytosolic glutamine synthetase β(1) gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants.Molecular genetics and genomics : MGG · 2012Article
- Glutamine synthetase in legumes: recent advances in enzyme structure and functional genomics.International journal of molecular sciences · 2012Review
- Glutamine synthetase is a molecular target of nitric oxide in root nodules of Medicago truncatula and is regulated by tyrosine nitration.Plant physiology · 2011Article
- Article
- Overexpressed glutamine synthetase gene modifies nitrogen metabolism and abiotic stress responses in rice.Plant cell reports · 2009Article
- Repercussion of mesophyll-specific overexpression of a soybean cytosolic glutamine synthetase gene in alfalfa (Plant science : an international journal of experimental plant biology · 2009Article
- Cloning and characterization of a cytosolic glutamine synthetase from Camellia sinensis (L.) O. Kuntze that is upregulated by ABA, SA, and H2O2.Molecular biotechnology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH4+ with glutanate to yield glutamine. Gene constructs consisting of the cauliflower mosaic virus (CaMV) 35S promoter driving a cytosolic isoform of GS (GS1) gene have been introduced into alfalfa (Medicago sativa). Although transcripts for the transgene were shown to accumulate to high levels in the leaves, they were undetectable in the nodules. However, significant amounts of beta-glucuronidase activity could be detected in nodules of plants containing the CaMV 35S promoter-beta-glucuronidase gene construct, suggesting that the transcript for the GS1 transgene is not stable in the root nodules. Leaves of alfalfa plants with the CaMV 35S promoter-GS1 gene showed high levels of accumulation of the transcript for the transgene when grown under low-nitrogen conditions and showed a significant drop in the level of GS1 transcripts when fed with high levels of NO3-. However, no increase in GS activity or polypeptide level was detected in the leaves of transgenic plants. The results suggest that GS1 is regulated at the level of RNA stability and protein turnover.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.