ArticleThe Journal of physiology2001
Voltage-dependent Ca2+ release in rat megakaryocytes requires functional IP3 receptors.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- The significance of interstitial cells in neurogastroenterology.Journal of neurogastroenterology and motility · 2014Article
- Expression and function of a T-type Ca2+ conductance in interstitial cells of Cajal of the murine small intestine.American journal of physiology. Cell physiology · 2014Article
- Novel consequences of voltage-dependence to G-protein-coupled P2Y1 receptors.British journal of pharmacology · 2008Article
- Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Metabotropic Ca(2+) channel-induced Ca(2+) release and ATP-dependent facilitation of arterial myocyte contraction.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Role of voltage-dependent modulation of store Ca2+ release in synchronization of Ca2+ oscillations.Biophysical journal · 2006Article
- Sensitivity limits for voltage control of P2Y receptor-evoked Ca2+ mobilization in the rat megakaryocyte.The Journal of physiology · 2004Article
- Ca2+ channel-sarcoplasmic reticulum coupling: a mechanism of arterial myocyte contraction without Ca2+ influx.The EMBO journal · 2003Article
- Properties of the demarcation membrane system in living rat megakaryocytes.Biophysical journal · 2003Article
- A theoretical model of slow wave regulation using voltage-dependent synthesis of inositol 1,4,5-trisphosphate.Biophysical journal · 2002Article
- Regenerative component of slow waves in the guinea-pig gastric antrum involves a delayed increase in [Ca(2+)](i) and Cl(-) channels.The Journal of physiology · 2002Article
- Depolarisation-evoked Ca2+ waves in the non-excitable rat megakaryocyte.The Journal of physiology · 2001Article
- Alkyl Mercury-Induced Toxicity: Multiple Mechanisms of Action.Reviews of environmental contamination and toxicologyReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using simultaneous whole-cell patch-clamp and fluorescence measurements of [Ca2+]i in rat megakaryocytes we have investigated the requirement for functional inositol 1,4,5-trisphosphate (IP3) receptors in Ca2+ release induced by membrane depolarization during agonist stimulation. Voltage-dependent Ca2+ release was observed during application of the IP3-generating agonists U46619 (a thromboxane A2 analogue) and ADP. Furthermore, voltage-dependent Ca2+ release was observed in the absence of exogenous agonist following sensitization of IP3 receptors with thimerosal. Depolarization-induced Ca2+ release was not detected during depletion of intracellular Ca2+ stores by thapsigargin. Thus, depletion of stores alone is not sufficient to confer voltage dependence upon the Ca2+ release mechanism. Block of IP3 receptors by carbacyclin-stimulated elevations in cAMP, uncaging of cAMP or exposure to a high concentration of caffeine reversibly abolished Ca2+ increases stimulated by both ADP and depolarization. The cAMP-dependent block was prevented by a peptide inhibitor of protein kinase A, indicating that an alteration of adenylate cyclase activity leading to modulation of protein kinase A activity does not underlie the control of Ca2+ release by voltage. These results are consistent with the requirement for functional IP3 receptors for voltage control of Ca2+ release from intracellular stores during inositol lipid signalling. The data also indicate the involvement of a voltage sensor downstream of surface membrane receptors in the depolarization-evoked Ca2+ response.
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