ArticleThe EMBO journal2001
In situ transcription and splicing in the Balbiani ring 3 gene.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Transcription Regulation Through Nascent RNA Folding.Journal of molecular biology · 2021Review
- Nascent RNA and the Coordination of Splicing with Transcription.Cold Spring Harbor perspectives in biology · 2019Review
- Long-read sequencing of nascent RNA reveals coupling among RNA processing events.Genome research · 2018Article
- Fractionation iCLIP detects persistent SR protein binding to conserved, retained introns in chromatin, nucleoplasm and cytoplasm.Nucleic acids research · 2017Article
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
- Perfect timing: splicing and transcription rates in living cells.Wiley interdisciplinary reviews. RNA · 2017Review
- Article
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- RNA-binding proteins in neurodegeneration: Seq and you shall receive.Trends in neurosciences · 2015Review
- Introduction to cotranscriptional RNA splicing.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Article
- How cells get the message: dynamic assembly and function of mRNA-protein complexes.Nature reviews. Genetics · 2013Review
- Nascent-seq indicates widespread cotranscriptional pre-mRNA splicing in Drosophila.Genes & development · 2011Article
- Real-time imaging of cotranscriptional splicing reveals a kinetic model that reduces noise: implications for alternative splicing regulation.The Journal of cell biology · 2011Article
- Review
- Dynamic histone acetylation is critical for cotranscriptional spliceosome assembly and spliceosomal rearrangements.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- RNA Polymerase II Elongation at the Crossroads of Transcription and Alternative Splicing.Genetics research international · 2011Article
- The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells.The Journal of cell biology · 2010Article
- Rational design of antisense oligomers to induce dystrophin exon skipping.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- The long journey of actin and actin-associated proteins from genes to polysomes.Cellular and molecular life sciences : CMLS · 2009Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Balbiani ring 3 (BR3) gene contains 38 introns, and more than half of them are co-transcriptionally excised. We have determined the in situ structure of the active BR3 gene by electron tomography. Each of the 20-25 nascent transcripts on the gene is present together with splicing factors and the RNA polymerase II in a nascent transcript and splicing complex, here called the NTS complex. The results indicate that extensive changes in overall shape, substructure and molecular mass take place repeatedly within an NTS complex as it moves along the gene. The volume and calculated mass of the NTS complexes show that, maximally, one complete spliceosome is assembled on the multi-intron transcript at any given time point. The structural data show that the spliceosome is not a structurally well-defined unit in situ and that the C-terminal domain of the elongating RNA polymerase II cannot carry spliceosomal components for all introns in the BR3 transcript. Our data indicate that spliceosomal factors are continuously added to and released from the NTS complexes during transcription elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.