ArticleThe EMBO journal2001
Regulation of glutamate dehydrogenase by reversible ADP-ribosylation in mitochondria.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 131 citations in OpenAlex.
- Restoring Homeostasis: Treating Amyotrophic Lateral Sclerosis by Resolving Dynamic Regulatory Instability.International journal of molecular sciences · 2025Article
- Evolution of Glutamate Metabolism viaInternational journal of molecular sciences · 2024Review
- Glutamine metabolism in diseases associated with mitochondrial dysfunction.Molecular and cellular neurosciences · 2023Review
- Epigenetic activation of secretory phenotypes in senescence by the FOXQ1-SIRT4-GDH signaling.Cell death & disease · 2023Article
- Regulation of tumor metabolism by post translational modifications on metabolic enzymes.Cancer gene therapy · 2023Review
- Amino acids downregulate SIRT4 to detoxify ammonia through the urea cycle.Nature metabolism · 2023Article
- Neuralized-like protein 4 (NEURL4) mediates ADP-ribosylation of mitochondrial proteins.The Journal of cell biology · 2022Article
- Alterations in Energy Metabolism, Mitochondrial Function and Redox Homeostasis in GK Diabetic Rat Tissues Treated with Aspirin.Life (Basel, Switzerland) · 2022Article
- A molecular toolbox for ADP-ribosyl binding proteins.Cell reports methods · 2021Article
- Physiological Role of Glutamate Dehydrogenase in Cancer Cells.Frontiers in oncology · 2020Article
- Role of Nicotinamide Adenine Dinucleotide and Related Precursors as Therapeutic Targets for Age-Related Degenerative Diseases: Rationale, Biochemistry, Pharmacokinetics, and Outcomes.Antioxidants & redox signaling · 2019Review
- Role of Sirtuins in Tumor Angiogenesis.Frontiers in oncology · 2019Review
- SIRT4 and Its Roles in Energy and Redox Metabolism in Health, Disease and During Exercise.Frontiers in physiology · 2019Review
- Resilient hepatic mitochondrial function and lack of iNOS dependence in diet-induced insulin resistance.PloS one · 2019Article
- Extracellular pyridine nucleotides as immune elicitors in arabidopsis.Plant signaling & behavior · 2017Article
- The Glutamate Dehydrogenase Pathway and Its Roles in Cell and Tissue Biology in Health and Disease.Biology · 2017Review
- Multiple Forms of Glutamate Dehydrogenase in Animals: Structural Determinants and Physiological Implications.Biology · 2016Review
- Sirtuins and the Metabolic Hurdles in Cancer.Current biology : CB · 2015Review
- Phenotypic and transcriptional profiling in Entamoeba histolytica reveal costs to fitness and adaptive responses associated with metronidazole resistance.Frontiers in microbiology · 2015Article
- Induction of Phosphoenolpyruvate Carboxykinase (PEPCK) during Acute Acidosis and Its Role in Acid Secretion by V-ATPase-Expressing Ionocytes.International journal of biological sciences · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial ADP-ribosylation leads to modification of two proteins of approximately 26 and 53 kDA: The nature of these proteins and, hence, the physiological consequences of their modification have remained unknown. Here, a 55 kDa protein, glutamate dehydrogenase (GDH), was established as a specific acceptor for enzymatic, cysteine-specific ADP-ribosylation in mitochondria. The modified protein was isolated from the mitochondrial preparation and identified as GDH by N-terminal sequencing and mass spectrometric analyses of tryptic digests. Incubation of human hepatoma cells with [14C]adenine demonstrated the occurrence of the modification in vivo. Purified GDH was ADP-ribosylated in a cysteine residue in the presence of the mitochondrial activity that transferred the ADP-ribose from NAD+ onto the acceptor site. ADP- ribosylation of GDH led to substantial inhibition of its catalytic activity. The stoichiometry between incorporated ADP-ribose and GDH subunits suggests that modification of one subunit per catalytically active homohexamer causes the inactivation of the enzyme. Isolated, ADP-ribosylated GDH was reactivated by an Mg2+-dependent mitochondrial ADP-ribosylcysteine hydrolase. GDH, a highly regulated enzyme, is the first mitochondrial protein identified whose activity may be modulated by ADP-ribosylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.