ArticleThe EMBO journal2001
Autoproteolysis and feedback in a protease cascade directing Drosophila dorsal-ventral cell fate.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Article
- Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation.PloS one · 2019Article
- Spatially Restricted Regulation of Spätzle/Toll Signaling during Cell Competition.Developmental cell · 2018Article
- Genome-Wide Host-Pathogen Interaction Unveiled by Transcriptomic Response of Diamondback Moth to Fungal Infection.PloS one · 2016Article
- Manduca sexta proprophenoloxidase activating proteinase-3 (PAP3) stimulates melanization by activating proPAP3, proSPHs, and proPOs.Insect biochemistry and molecular biology · 2014Article
- A ventrally localized protease in the Drosophila egg controls embryo dorsoventral polarity.Current biology : CB · 2012Article
- Cloning and the expression pattern of Spätzle gene during embryonic development and bacterial challenge in Artemia sinica.Molecular biology reports · 2012Article
- Molecular mechanisms of EGF signaling-dependent regulation of pipe, a gene crucial for dorsoventral axis formation in Drosophila.Development genes and evolution · 2012Article
- Maternal Wnt/β-catenin signaling coactivates transcription through NF-κB binding sites during Xenopus axis formation.PloS one · 2012Article
- Structure-function analysis of grass clip serine protease involved in Drosophila Toll pathway activation.The Journal of biological chemistry · 2011Article
- Endocytosis is required for Toll signaling and shaping of the Dorsal/NF-kappaB morphogen gradient during Drosophila embryogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Activation of Snake in a serine protease cascade that defines the dorsoventral axis is atypical and pipe-independent in Drosophila embryos.FEBS letters · 2010Article
- Pipe-dependent ventral processing of Easter by Snake is the defining step in Drosophila embryo DV axis formation.Current biology : CB · 2010Article
- Distinct functional specificities are associated with protein isoforms encoded by the Drosophila dorsal-ventral patterning gene pipe.Development (Cambridge, England) · 2009Article
- A single modular serine protease integrates signals from pattern-recognition receptors upstream of the Drosophila Toll pathway.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Gastrulation defective protease interacts with anionic components of the Drosophila ovary extracellular matrix.Protein and peptide letters · 2009Article
- A positive feedback mechanism in the Manduca sexta prophenoloxidase activation system.Insect biochemistry and molecular biology · 2008Article
- Mutagenesis of the cysteine-rich clip domain in the Drosophila patterning protease, Snake.Archives of biochemistry and biophysics · 2008Article
- Spatially dependent activation of the patterning protease, Easter.FEBS letters · 2006Article
- Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three serine protease zymogens, Gastrulation defective (GD), Snake (Snk) and Easter (Ea), and a nerve growth factor-like growth factor ligand precursor, Spaetzle, are required for specification of dorsal- ventral cell fate during Drosophila embryogenesis. The proteases have been proposed to function in a sequential activation cascade within the extracellular compartment called the perivitelline space. We examined biochemical interactions between these four proteins using a heterologous co-expression system. The results indicate that the three proteases do function in a sequential activation cascade, that GD becomes active and initiates the cascade and that interaction between GD and Snk is sufficient for GD to cleave itself autoproteolytically. The proteolytically active form of Ea cleaves GD at a different position, revealing biochemical feedback in the pathway. Both GD and Snk bind to heparin-Sepharose, providing a link between the pipe-defined ventral prepattern and the protease cascade. Our results suggest a model of the cascade in which initiation is by relief from inhibition, and spatial regulation of activity is due to interaction with sulfated proteoglycans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.