ArticleBritish journal of pharmacology2001
Transfection of annexin 1 in monocytic cells produces a high degree of spontaneous and stimulated apoptosis associated with caspase-3 activation.
Article in British journal of pharmacology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 114 citations in OpenAlex.
- Annexin A1 affects tumor metastasis through epithelial-mesenchymal transition: a narrative review.Translational cancer research · 2022Review
- Bixafen causes cardiac toxicity in zebrafish (Danio rerio) embryos.Environmental science and pollution research international · 2021Article
- SNPs in miRNAs and Target Sequences: Role in Cancer and Diabetes.Frontiers in genetics · 2021Review
- ROS-Mediated Apoptosis and Autophagy in Ovarian Cancer Cells Treated with Peanut-Shaped Gold Nanoparticles.International journal of nanomedicine · 2021Article
- Evaluation of phytoestrogens in inducing cell death mediated by decreasing Annexin A1 in Annexin A1-knockdown leukemia cells.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2020Article
- Annexin-A1: Therapeutic Potential in Microvascular Disease.Frontiers in immunology · 2019Review
- Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol.Oncology letters · 2018Article
- Annexin A1: Uncovering the Many Talents of an Old Protein.International journal of molecular sciences · 2018Review
- Ceramide-Protein Interactions Modulate Ceramide-Associated Lipotoxic Cardiomyopathy.Cell reports · 2018Article
- Functional Association between Regulatory RNAs and the Annexins.International journal of molecular sciences · 2018Review
- Influenza A virus enhances its propagation through the modulation of Annexin-A1 dependent endosomal trafficking and apoptosis.Cell death and differentiation · 2016Article
- Factors controlling permeability of the blood-brain barrier.Cellular and molecular life sciences : CMLS · 2016Review
- Anticancer activity of bicalutamide-loaded PLGA nanoparticles in prostate cancers.Experimental and therapeutic medicine · 2015Article
- Annexin-1 regulated by HAUSP is essential for UV-induced damage response.Cell death & disease · 2015Article
- Analysis of protein expression profiles in the thymus of chickens infected with Marek's disease virus.Virology journal · 2012Article
- The involvement of anti-inflammatory protein, annexin A1, in ocular toxoplasmosis.Molecular vision · 2012Article
- Cell signaling by protein carbonylation and decarbonylation.Antioxidants & redox signaling · 2010Review
- Effects of exogenous annexin-1 on lipopolysaccharide-induced proliferation and reactive oxygen species production partially through modulation of CRAC channels but independent of NF-kappaB pathway.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2009Article
- MicroRNA-196a targets annexin A1: a microRNA-mediated mechanism of annexin A1 downregulation in cancers.Oncogene · 2008Article
- Annexin-A1: a pivotal regulator of the innate and adaptive immune systems.British journal of pharmacology · 2008Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transfection of the pre-monomyelocytic U937 cell line with a plasmid coding for full-length annexin 1 (ANX1, 347 amino acid) leads to cell death by promoting apoptosis. In addition, over-expression of the N-terminal and the first domain of the protein (144 amino acids, clone ANX1-S), which does not contain the Ca2+ binding sites, gives susceptibility to cell apoptosis following activation by either 5 ng ml(-1) tumour necrosis factor (TNF)-alpha or 1 - 40 microg ml-1 etoposide. This was demonstrated by using the fluorescent labelled annexin V, cell cycle and nuclear staining analyses. Transfection with an empty plasmid (clone CMV) or with a plasmid carrying the cDNA antisense for ANX1 (clone ANX1-AS) did not alter U937 cells to the degree of apoptosis promoted by either stimulant. Treatment of CMV U937 cells with TNF-alpha increased ANX1 mRNA and protein expression in a time-dependent manner, with maximal increases at 3 and 6 h, respectively. Clone ANX1-S showed higher constitutive (more than 2 fold) and activated caspase-3 activity, associated with higher phospholipase A2 (PLA2) activity (in the region of +50 - 100%), whereas expression of cytosolic PLA2 Bax and Bcl-2 were similar in all cell clones, as determined by Western blotting. In conclusion, this study demonstrates a complex regulatory role of cell apoptosis for ANX1, at least with regards to cells of the myelo-monocytic lineage.
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