ArticleInfection and immunity2001
Improved pattern for genome-based screening identifies novel cell wall-attached proteins in gram-positive bacteria.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 68 citations in OpenAlex.
- Article
- Genetics, Structure, and Function of Group A Streptococcal Pili.Frontiers in microbiology · 2021Review
- Molecular Cloning and Immunogenicity Evaluation of PpiC, GelE, and VS87_01105 Proteins of Enterococcus faecalis as Vaccine CandidatesIranian biomedical journal · 2019Article
- Structure and function of α-glucan debranching enzymes.Cellular and molecular life sciences : CMLS · 2016Review
- Two Putative Polysaccharide Deacetylases Are Required for Osmotic Stability and Cell Shape Maintenance in Bacillus anthracis.The Journal of biological chemistry · 2015Article
- Clostridium difficile has a single sortase, SrtB, that can be inhibited by small-molecule inhibitors.BMC microbiology · 2014Article
- An exhaustive yet simple virtual screening campaign against Sortase A from multiple drug resistant Staphylococcus aureus.Molecular biology reports · 2014Article
- Identification of peptidoglycan-associated proteins as vaccine candidates for enterococcal infections.PloS one · 2014Article
- An automatic method for identifying surface proteins in bacteria: SLEP.BMC bioinformatics · 2010Article
- Comprehensive evaluation of Streptococcus sanguinis cell wall-anchored proteins in early infective endocarditis.Infection and immunity · 2009Article
- Identification of novel LPXTG-linked surface proteins from Streptococcus gordonii.Microbiology (Reading, England) · 2009Article
- Identification and experimental verification of protective antigens against Streptococcus suis serotype 2 based on genome sequence analysis.Current microbiology · 2009Article
- Overcoming function annotation errors in the Gram-positive pathogen Streptococcus suis by a proteomics-driven approach.BMC genomics · 2008Article
- Five genes encoding surface-exposed LPXTG proteins are enriched in hospital-adapted Enterococcus faecium clonal complex 17 isolates.Journal of bacteriology · 2007Article
- Structure of the streptococcal cell wall C5a peptidase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Genome-wide detection and analysis of cell wall-bound proteins with LPxTG-like sorting motifs.Journal of bacteriology · 2005Article
- Binding of alpha2-macroglobulin to GRAB (Protein G-related alpha2-macroglobulin-binding protein), an important virulence factor of group A streptococci, is mediated by two charged motifs in the DeltaA region.The Biochemical journal · 2004Article
- A comparative genome analysis identifies distinct sorting pathways in gram-positive bacteria.Infection and immunity · 2004Article
- Genome-based bioinformatic selection of chromosomal Bacillus anthracis putative vaccine candidates coupled with proteomic identification of surface-associated antigens.Infection and immunity · 2003Article
- Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution.Genome research · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With a large number of sequenced microbial genomes available, tools for identifying groups or classes of proteins have become increasingly important. Here we present an improved pattern for the identification of cell wall-attached proteins (CWPs), a group of proteins with diverse and important functions in gram-positive bacteria. This tripartite pattern is based on analysis of 65 previously described cell wall-attached proteins and takes into account the three principal requirements for cell wall sorting; a sortase target region (LPXTGX), a membrane-spanning region, and a charged stop-transfer tail. In five different genomes of gram-positive bacteria, the tripartite pattern identified a total of 35 putative CWPs, 19 of which were novel. The specificity and sensitivity of the tripartite pattern are higher than those of the classical pattern, which is based solely on the sortase target region. Several putative CWPs with atypical sortase target regions were identified. In the complete genome of the important human pathogen Streptococcus pyogenes, the tripartite pattern identified 14 putative CWPs. Seven of the putative S. pyogenes proteins were novel, and two of these were a 5' nucleotidase and a pullulanase. This study represents the first whole-genome screening for CWPs, and we conclude that the tripartite pattern is highly suitable for this purpose. Identification of CWPs using this pattern offers important possibilities in the study of the pathogenesis and physiology of gram-positive bacteria.
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