ArticleInfection and immunity2001
Intracellular survival of Brucella spp. in human monocytes involves conventional uptake but special phagosomes.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 74 citations in OpenAlex.
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- Scenario of pathogenesis and socioeconomic burden of human brucellosis in Saudi Arabia.Saudi journal of biological sciences · 2021Review
- Cathepsins in Bacteria-Macrophage Interaction: Defenders or Victims of Circumstance?Frontiers in cellular and infection microbiology · 2020Review
- Binding of Brucella protein, Bp26, to select extracellular matrix molecules.BMC molecular and cell biology · 2019Article
- Targeting redox heterogeneity to counteract drug tolerance in replicatingScience translational medicine · 2019Article
- Circulating Th1, Th2, Th17, Treg, and PD-1 Levels in Patients with Brucellosis.Journal of immunology research · 2019Article
- Influence of platelet-activating factor receptor (PAFR) on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus.BMC microbiology · 2016Article
- Early transcriptional responses of internalization defective Brucella abortus mutants in professional phagocytes, RAW 264.7.BMC genomics · 2013Article
- Host cell autophagy in immune response to zoonotic infections.Clinical & developmental immunology · 2012Review
- Brucella melitensis cyclic di-GMP phosphodiesterase BpdA controls expression of flagellar genes.Journal of bacteriology · 2011Article
- Interactions of the human pathogenic Brucella species with their hosts.Annual review of microbiology · 2011Review
- Brucella melitensis, B. neotomae and B. ovis elicit common and distinctive macrophage defense transcriptional responses.Experimental biology and medicine (Maywood, N.J.) · 2009Article
- Survival of the fittest: how Brucella strains adapt to their intracellular niche in the host.Medical microbiology and immunology · 2009Review
- Evidence of Brucella abortus OPS dictating uptake and restricting NF-kappaB activation in murine macrophages.Microbes and infection · 2008Article
- "Alternative" endocytic mechanisms exploited by pathogens: new avenues for therapeutic delivery?Advanced drug delivery reviews · 2007Review
- Role of urease in megasome formation and Helicobacter pylori survival in macrophages.Journal of leukocyte biology · 2006Article
- Opsonized virulent Brucella abortus replicates within nonacidic, endoplasmic reticulum-negative, LAMP-1-positive phagosomes in human monocytes.Infection and immunity · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella spp. are facultative intracellular parasites of various mammals, including humans, typically infecting lymphoid as well as reproductive organs. We have investigated how B. suis and B. melitensis enter human monocytes and in which compartment they survive. Peripheral blood monocytes readily internalized nonopsonized brucellae and killed most of them within 12 to 18 h. The presence of Brucella-specific antibodies (but not complement) increased the uptake of bacteria without increasing their intracellular survival, whereas adherence of the monocytes or incubation in Ca(2+)- and Mg(2+)-free medium reduced the uptake. Engulfment of all Brucella organisms (regardless of bacterial viability or virulence) initially resulted in phagosomes with tightly apposed walls (TP). Most TP were fully fusiogenic and matured to spacious phagolysosomes containing degraded bacteria, whereas some TP (more in monocyte-derived macrophages, HeLa cells, and CHO cells than in monocytes) remained tightly apposed to intact bacteria. Immediate treatment of infected host cells with the lysosomotropic base ammonium chloride caused a swelling of all phagosomes and a rise in the intraphagosomal pH, abolishing the intracellular survival of Brucella. These results indicate that (i) human monocytes readily internalize Brucella in a conventional way using various phagocytosis-promoting receptors, (ii) the maturation of some Brucella phagosomes is passively arrested between the steps of acidification and phagosome-lysosome fusion, (iii) brucellae are killed in maturing but not in arrested phagosomes, and (iv) survival of internalized Brucella depends on an acidic intraphagosomal pH and/or close contact with the phagosomal wall.
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Registered trials
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