ArticleInfection and immunity2001
Characterization of the groESL operon in Listeria monocytogenes: utilization of two reporter systems (gfp and hly) for evaluating in vivo expression.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 72 citations in OpenAlex.
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- Fitness Trade-Offs Determine the Role of the Molecular Chaperonin GroEL in Buffering Mutations.Molecular biology and evolution · 2015Article
- Identification of a ferritin-like protein of Listeria monocytogenes as a mediator of β-lactam tolerance and innate resistance to cephalosporins.BMC microbiology · 2012Article
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- Transcriptome analysis of alkali shock and alkali adaptation in Listeria monocytogenes 10403S.Foodborne pathogens and disease · 2010Article
- In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection.PLoS pathogens · 2009Article
- Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S.BMC microbiology · 2008Article
- Genome-wide screen for temperature-regulated genes of the obligate intracellular bacterium, Rickettsia typhi.BMC microbiology · 2008Article
- Comparative analysis of the sigma B-dependent stress responses in Listeria monocytogenes and Listeria innocua strains exposed to selected stress conditions.Applied and environmental microbiology · 2008Article
- Transcriptomic and phenotypic analyses suggest a network between the transcriptional regulators HrcA and sigmaB in Listeria monocytogenes.Applied and environmental microbiology · 2007Article
- Microarray-based characterization of the Listeria monocytogenes cold regulon in log- and stationary-phase cells.Applied and environmental microbiology · 2007Article
- Low concentrations of bile salts induce stress responses and reduce motility in Bacillus cereus ATCC 14579 [corrected].Journal of bacteriology · 2007Article
- Identification of in vivo-expressed immunogenic proteins by serological proteome analysis of the Bacillus anthracis secretome.Infection and immunity · 2007Article
- Identification of small Hfq-binding RNAs in Listeria monocytogenes.RNA (New York, N.Y.) · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of intracellular pathogens to sense and adapt to the hostile environment of the host is an important factor governing virulence. We have sequenced the operon encoding the major heat shock proteins GroES and GroEL in the gram-positive food-borne pathogen Listeria monocytogenes. The operon has a conserved orientation in the order groES groEL. Upstream of groES and in the opposite orientation is a gene encoding a homologue of the Bacillus subtilis protein YdiL, while downstream of groEL is a gene encoding a putative bile hydrolase. We used both reverse transcriptase-PCR (RT-PCR) and transcriptional fusions to the UV-optimized Aequorea victoria green fluorescent protein (GFP(UV)) to analyze expression of groESL under various environmental stress conditions, including heat shock, ethanol stress, and acid shock, and during infection of J774 mouse macrophage cells. Strains harboring GFP(UV) transcriptional fusions to the promoter region of groESL demonstrated a significant increase in fluorescence following heat shock that was detected by both fluorimetry and fluorescence microscopy. Using both RT-PCR and GFP technology we detected expression of groESL following internalization by J774 cells. Increased intracellular expression of dnaK was also determined using RT-PCR. We have recently described a system which utilizes L. monocytogenes hemolysin as an in vivo reporter of gene expression within the host cell phagosome (C. G. M. Gahan and C. Hill, Mol. Microbiol. 36:498-507, 2000). In this study a strain was constructed in which hemolysin expression was placed under the control of the groESL promoter. In this strain hemolysin expression during infection also confirms transcription from the groESL promoter during J774 and murine infection, albeit at lower levels than the known virulence factor plcA.
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