ArticleRNA (New York, N.Y.)2001
Optimization and optimality of a short ribozyme ligase that joins non-Watson-Crick base pairings.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit.Biology · 2023Article
- Acquisition of Dual Ribozyme-Functions in Nonfunctional Short Hairpin RNAs through Kissing-Loop Interactions.Life (Basel, Switzerland) · 2022Article
- Lipid membranes modulate the activity of RNA through sequence-dependent interactions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Strategies for Covalent Labeling of Long RNAs.Chembiochem : a European journal of chemical biology · 2021Review
- tRNA sequences can assemble into a replicator.eLife · 2021Article
- In vitro selections with RNAs of variable length converge on a robust catalytic core.Nucleic acids research · 2021Article
- In vitro selection of ribozyme ligases that use prebiotically plausible 2-aminoimidazole-activated substrates.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Systematic minimization of RNA ligase ribozyme through large-scale design-synthesis-sequence cycles.Nucleic acids research · 2019Article
- Molecular trade-offs in RNA ligases affected the modular emergence of complex ribozymes at the origin of life.Royal Society open science · 2017Article
- Prebiotic Factors Influencing the Activity of a Ligase Ribozyme.Life (Basel, Switzerland) · 2017Article
- Hypercycle.PLoS computational biology · 2016Article
- Reconciling ligase ribozyme activity with Fatty Acid vesicle stability.Life (Basel, Switzerland) · 2014Article
- Article
- In vitro selection using modified or unnatural nucleotides.Current protocols in nucleic acid chemistry · 2014Review
- Mapping L1 ligase ribozyme conformational switch.Journal of molecular biology · 2012Article
- Mechanisms of RNA catalysis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Article
- Identification of dynamical hinge points of the L1 ligase molecular switch.RNA (New York, N.Y.) · 2010Article
- Structure-guided engineering of the regioselectivity of RNA ligase ribozymes.Journal of the American Chemical Society · 2009Article
- In vitro selection of a 5'-purine ribonucleotide transferase ribozyme.Nucleic acids research · 2007Article
- De novo synthesis and development of an RNA enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A small ribozyme ligase (L1) selected from a random sequence population appears to utilize non-Watson-Crick base pairs at its ligation junction. Mutational and selection analyses confirmed the presence of these base pairings. Randomization of the L1 core and selection of active ligases yielded highly active variants whose rates were on the order of 1 min(-1). Base-pairing covariations confirmed the general secondary structure of the ligase, and the most active ligases contained a novel pentuple sequence covariation. The optimized L1 ligases may be optimal within their sequence spaces, and minimal ligases that span less than 60 nt in length have been engineered based on these results.
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