ArticleJournal of bacteriology2001
Evidence against an Interaction between the mRNA downstream box and 16S rRNA in translation initiation.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 63 citations in OpenAlex.
- Leaderless mRNAs in the Spotlight: Ancient but Not Outdated!Microbiology spectrum · 2018Review
- Comparative genomic analysis of translation initiation mechanisms for genes lacking the Shine-Dalgarno sequence in prokaryotes.Nucleic acids research · 2017Article
- Ribosome heterogeneity: another level of complexity in bacterial translation regulation.Current opinion in microbiology · 2013Review
- Multifactorial determinants of protein expression in prokaryotic open reading frames.Journal of molecular biology · 2010Article
- Dynamic evolution of translation initiation mechanisms in prokaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Translation initiation complex formation in the crenarchaeon Sulfolobus solfataricus.RNA (New York, N.Y.) · 2009Article
- An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis?Molecular cell · 2009Article
- Saturation mutagenesis of a +1 programmed frameshift-inducing mRNA sequence derived from a yeast retrotransposon.RNA (New York, N.Y.) · 2007Article
- Efficient expression of gene variants that harbour AGA codons next to the initiation codon.Nucleic acids research · 2007Article
- Development of novel types of plastid transformation vectors and evaluation of factors controlling expression.Transgenic research · 2005Article
- Initiation of protein synthesis in bacteria.Microbiology and molecular biology reviews : MMBR · 2005Review
- A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli.Nucleic acids research · 2004Article
- Translation initiation with 70S ribosomes: an alternative pathway for leaderless mRNAs.Nucleic acids research · 2004Article
- In vivo evidence for the prokaryotic model of extended codon-anticodon interaction in translation initiation.The EMBO journal · 2003Article
- Protein S1 counteracts the inhibitory effect of the extended Shine-Dalgarno sequence on translation.RNA (New York, N.Y.) · 2002Article
- Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on the complementarity of the initial coding region (downstream box [db]) of several bacterial and phage mRNAs to bases 1469 to 1483 in helix 44 of 16S rRNA (anti-downstream box [adb]), it has been proposed that db-adb base pairing enhances translation in a way that is similar to that of the Shine-Dalgarno (SD)/anti-Shine-Dalgarno (aSD) interaction. Computer modeling of helix 44 on the 30S subunit shows that the topography of the 30S ribosome does not allow a simultaneous db-adb interaction and placement of the initiation codon in the ribosomal P site. Thus, the db-adb interaction cannot substitute for the SD-aSD interaction in translation initiation. We have always argued that any contribution of the db-adb interaction should be most apparent on mRNAs devoid of an SD sequence. Here, we show that 30S ribosomes do not bind to leaderless mRNA in the absence of initiator tRNA, even when the initial coding region shows a 15-nucleotide complementarity (optimal fit) with the putative adb. In addition, an optimized db did not affect the translational efficiency of a leaderless lambda cI-lacZ reporter construct. Thus, the db-adb interaction can hardly serve as an initial recruitment signal for ribosomes. Moreover, we show that different leaderless mRNAs are translated in heterologous systems although the sequence of the putative adb's within helix 44 of the 30S subunits of the corresponding bacteria differ largely. Taken our data together with those of others (M. O'Connor, T. Asai, C. L. Squires, and A. E. Dahlberg, Proc. Natl. Acad. Sci. USA 96:8973-8978, 1999; A. La Teana, A. Brandi, M. O'Connor, S. Freddi, and C. L. Pon, RNA 6:1393-1402, 2000), we conclude that the db does not base pair with the adb.
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