ArticleThe Plant cell2001
Influence of KDEL on the fate of trimeric or assembly-defective phaseolin: selective use of an alternative route to vacuoles.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 80 citations in OpenAlex.
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- Two γ-zeins induce the unfolded protein response.Plant physiology · 2021Article
- A Model for ERD2 Function in Higher Plants.Frontiers in plant science · 2020Review
- The pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in Arabidopsis thaliana plants.World journal of microbiology & biotechnology · 2016Article
- Phaseolin expression in tobacco chloroplast reveals an autoregulatory mechanism in heterologous protein translation.Plant biotechnology journal · 2016Article
- Protein body formation in the endoplasmic reticulum as an evolution of storage protein sorting to vacuoles: insights from maize γ-zein.Frontiers in plant science · 2014Article
- Unconventional pathways of secretory plant proteins from the endoplasmic reticulum to the vacuole bypassing the Golgi complex.Plant signaling & behavior · 2013Review
- Auxiliary KChIP4a suppresses A-type K+ current through endoplasmic reticulum (ER) retention and promoting closed-state inactivation of Kv4 channels.The Journal of biological chemistry · 2013Article
- BINDING PROTEIN is a master regulator of the endoplasmic reticulum stress sensor/transducer bZIP28 in Arabidopsis.The Plant cell · 2013Article
- Traffic of human α-mannosidase in plant cells suggests the presence of a new endoplasmic reticulum-to-vacuole pathway without involving the Golgi complex.Plant physiology · 2013Article
- Comparative study of wheat low-molecular-weight glutenin and α-gliadin trafficking in tobacco cells.Plant cell reports · 2013Article
- Localization of rabies virus glycoprotein into the endoplasmic reticulum produces immunoprotective antigen.The protein journal · 2012Article
- Influence of an ER-retention signal on the N-glycosylation of recombinant human α-L-iduronidase generated in seeds of Arabidopsis.Plant molecular biology · 2012Article
- Secretory pathway research: the more experimental systems the better.The Plant cell · 2012Review
- A new type of compartment, defined by plant-specific Atg8-interacting proteins, is induced upon exposure of Arabidopsis plants to carbon starvation.The Plant cell · 2012Article
- Expression of a new chimeric protein with a highly repeated sequence in tobacco cells.Plant cell reports · 2011Article
- A plant secretory signal peptide targets plastome-encoded recombinant proteins to the thylakoid membrane.Plant molecular biology · 2011Article
- Higher accumulation of F1-V fusion recombinant protein in plants after induction of protein body formation.Plant molecular biology · 2010Article
- Induction of protein body formation in plant leaves by elastin-like polypeptide fusions.BMC biology · 2009Article
- Plant-based strategies aimed at expressing HIV antigens and neutralizing antibodies at high levels. Nef as a case study.Transgenic research · 2009Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tetrapeptide KDEL is commonly found at the C terminus of soluble proteins of the endoplasmic reticulum (ER), and it contributes to their localization by interacting with a receptor that recycles between the Golgi complex and the ER. We investigated the effects of the addition of KDEL to phaseolin, a protein normally delivered from the ER to storage vacuoles via the Golgi complex. We show that KDEL prevents acquisition of trans-Golgi-specific glycan modifications and causes interactions with the chaperone BiP that are distinct from the ones between BiP and defective proteins. KDEL markedly increases the stability of phaseolin, but a small proportion of phaseolin-KDEL slowly reaches the vacuole without undergoing Golgi-mediated glycan modifications, in a process that can be inhibited by brefeldin A but not monensin. Our results indicate that KDEL can operate with high efficiency before proteins can reach the late Golgi cisternae but allows or promotes delivery to vacuoles via an alternative mechanism. However, addition of KDEL does not alter the destiny of an assembly-defective form of phaseolin, suggesting that the plant ER quality control mechanism is dominant over KDEL effects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.